KRAS Antibody (OTI1D1)

Novus Biologicals | Catalog # NBP2-45535

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D1
View all KRAS Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human KRAS (NP_203524) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KRAS Antibody (OTI1D1)

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Western Blot: KRAS Antibody (OTI1D1) [NBP2-45535]

Western Blot: KRAS Antibody (OTI1D1) [NBP2-45535]

Western Blot: KRAS Antibody (OTI1D1) [NBP2-45535] - Total cell lysates from human cancer cell lines PC-3, HeLa and HT-29 cells were analyzed by Western blot. PVDF membrane was probed with Human KRAS (OTI1D1) Antibody (NBP2-45535). A specific band was detected for KRAS at approximately 21 kDa. Image from verified customer review.

Applications for KRAS Antibody (OTI1D1)

Application
Recommended Usage

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-45535 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KRAS

KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Alternate Names

cellular c-Ki-ras2 proto-oncogene, c-Ki-ras, c-K-ras, GTPase KRas, KI-RAS, Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog, K-Ras 2, K-ras p21 protein, KRAS1, K-RAS2A, K-RAS2B, KRAS2NS3, K-RAS4A, K-RAS4B, NS, oncogene KRAS2, PR310 c-K-ras oncogene, RASK2c-Kirsten-ras protein, transforming protein p21, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Gene Symbol

KRAS

Additional KRAS Products

Product Documents for KRAS Antibody (OTI1D1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KRAS Antibody (OTI1D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for KRAS Antibody (OTI1D1) (1)

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  • KRAS Antibody (OTI1D1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 3 human cancer cell lines
    Species: Human
    Verified Customer | Posted 07/17/2018
    Total cell lysates from PC-3,HeLa and HT-29 cells were subjected to western blot. PVDF membrane were probed with 1 mm/ml Human KRAS Antibody (OTI1D1) (NBP2-45535). A specific band was detected for KRAS at approximately 21 kDa. This experiment was conducted under reducing conditions
    KRAS Antibody (OTI1D1) NBP2-45535

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