Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ku80/XRCC5 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to residues 323-338 [FSKVDEEQMKYKSEGK] of the 80 kDa Ku80 protein.

Reactivity Notes

This antibody reacts with human, rat, and mouse.

Specificity

Reacts with residues 323-338 [FSKVDEEQMKYKSEGK] of the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ku80/XRCC5 Antibody - BSA Free

Immunoprecipitation: Ku80/XRCC5 Antibody - BSA Free [NB100-503]

Immunoprecipitation: Ku80/XRCC5 Antibody - BSA Free [NB100-503]

Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-503] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-503. A Ku80 deficient cell lysate, XRS, was used as a negative control.

Applications for Ku80/XRCC5 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody has only been tested in Western blot. Recommended working dilution: WB at 1:500 dilution However, the investigator should determine the optimal dilution.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ku80/XRCC5

Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance. The Ku heterodimer functions at two kinds of DNA ends: telomeres and double-strand breaks. The role that Ku plays at these two classes of termini must be distinct, because Ku is required for accurate and efficient joining of double-strand breaks while similar DNA repair events are normally prohibited at chromosome ends.

Long Name

Lupus Ku Autoantigen Protein 86

Alternate Names

CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5

Entrez Gene IDs

7520 (Human); 22596 (Mouse); 363247 (Rat)

Gene Symbol

XRCC5

Additional Ku80/XRCC5 Products

Product Documents for Ku80/XRCC5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ku80/XRCC5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ku80/XRCC5 Antibody - BSA Free

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