Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ku80/XRCC5 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to residues 419-440 LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein.

Specificity

Reacts with residues 419-440 [LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ku80/XRCC5 Antibody - BSA Free

Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]

Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]

Western Blot: Ku80/XRCC5 Antibody [NB100-508] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. KU80 (NB100-508) primary antibody at 1:2000 dilution, 4C, overnight. Western blot image submitted by a verified customer review.
Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]

Western Blot: Ku80/XRCC5 AntibodyBSA Free [NB100-508]

Western Blot: Ku80/XRCC5 Antibody [NB100-508] - Analysis of Ku80 using NB100-508 at 1:500 dilution on 50 ug of total lysates of A431 (lane 1) and AR42J (lane 2) cells.
Immunoprecipitation: Ku80/XRCC5 Antibody - BSA Free [NB100-508]

Immunoprecipitation: Ku80/XRCC5 Antibody - BSA Free [NB100-508]

Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-508] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-508. A Ku80 deficient cell lysate, XRS, was used as a negative control.

Applications for Ku80/XRCC5 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618) This antibody has only been tested in Western blot.

Reviewed Applications

Read 1 review rated 5 using NB100-508 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ku80/XRCC5

Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance. The Ku heterodimer functions at two kinds of DNA ends: telomeres and double-strand breaks. The role that Ku plays at these two classes of termini must be distinct, because Ku is required for accurate and efficient joining of double-strand breaks while similar DNA repair events are normally prohibited at chromosome ends.

Long Name

Lupus Ku Autoantigen Protein 86

Alternate Names

CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5

Entrez Gene IDs

7520 (Human); 22596 (Mouse); 363247 (Rat)

Gene Symbol

XRCC5

Additional Ku80/XRCC5 Products

Product Documents for Ku80/XRCC5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ku80/XRCC5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ku80/XRCC5 Antibody - BSA Free

Customer Reviews for Ku80/XRCC5 Antibody - BSA Free (1)

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Customer Images

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  • Ku80/XRCC5 Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: cell line, whole cell lysate and 20 ug whole cell lysate
    Species: Human and Mouse
    Verified Customer | Posted 10/10/2020
    Western Blot: MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates were loaded with 20 ug/lane. 10% SDS-PAGE. KU80 (NB100-508) primary antibody: 1:2000, 4℃, overnight.
    Ku80/XRCC5 Antibody - BSA Free NB100-508

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