L-trans-2,4-PDC

Key Product Details

Product Description

L-trans-2,4-PDC is a potent, competitive, transportable EAAT1-4 inhibitor/non-transportable EAAT5 inhibitor. In [³H]-d-Asp uptake assays in HEK293 cells expressing human EAAT1, EAAT2 and EAAT3, K_i values are 20, 20 and 109 μM, respectively. In a FLIPR Membrane Potential (FMP) assay, K_m values for L-trans-2,4-PDC are 7.7, 11 and 19 μM for human EAAT2, EAAT3 and EAAT1, respectively.

Product Specifications for L-trans-2,4-PDC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for L-trans-2,4-PDC

The following data is based on the product molecular weight 159.14.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	6.28 mL	31.42 mL	62.84 mL
5 mM	1.26 mL	6.28 mL	12.57 mL
10 mM	0.63 mL	3.14 mL	6.28 mL
50 mM	0.13 mL	0.63 mL	1.26 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 8 publications citing the usage of this product.

• Jensen and Bräuner-Osborne Pharmacological characterization of human excitatory amino acid transporters EAAT1, EAAT2 and EAAT3 in a fluorescence-based membrane potential assay. Biochem.Pharmacol. 2004 PMID: 15135308
• Mitrovic and Johnston Regional differences in the inhibition of L-glutamate amd L-aspartate sodium-dependent high affinity uptake systems in rat CNS synaptosomes by L-trans-pyrrolidine-2,4-dicarboxylic threo-3-hydroxy-D-aspartate and D-aspartate. Neurochem.Int. 1994 PMID: 7981641
• Zuiderwijk Effects of uptake carrier blockers SK & F 89976-A and L-trans-PDC on in vivo release of amino acids in rat hippocampus. Eur.J.Pharmacol. 1996 PMID: 8836615
• Bridges Conformationally defined neurotransmitter analogues. Selective inhibition of glutamate uptake by one pyrrolidine-2,4-dicarboxylate diastereomer. J.Med.Chem. 1991 PMID: 1671706

Product Documents for L-trans-2,4-PDC