Human Lambda Light Chain ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Human Lambda Light Chain ELISA Kit (Colorimetric)

Kit Contents for Human Lambda Light Chain ELISA Kit (Colorimetric)

• Adhesive Strip (For 96 wells)
• Assay plate
• HRP-conjugate (100 x concentrate)
• HRP-conjugate Diluent
• Instruction manual
• Sample Diluent
• Standard (Freeze dried)
• Stop Solution
• TMB Substrate
• Wash Buffer (25 x concentrate)

Preparation and Storage

Background: Lambda Light Chain

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30, the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Additional Lambda Light Chain Products

• All Products for Lambda Light Chain
• Lambda Light Chain ELISA Kits
• Lambda Light Chain Primary Antibodies
• Lambda Light Chain Secondary Antibodies

Product Documents for Human Lambda Light Chain ELISA Kit (Colorimetric)