LMW-PTP/ACP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30788

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

BSA Free
View all LMW-PTP/ACP1 Primary Antibodies »

Product Specifications

Immunogen

The human protein, LMW-PTP/ACP1 (GB Accession #M83653) was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for LMW-PTP/ACP1 Antibody - BSA Free

LMW-PTP/ACP1 Antibody

Western Blot: LMW-PTP/ACP1 Antibody [NBP3-30788] -

Western Blot: LMW-PTP/ACP1 Antibody [NBP3-30788] - CHO cells were transfected with either Vector (pSRa) or expression vector driving expression of HCPTPA (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2mg of lysate protein was incubated for 1 h with 10ml of sheep-anti-LMWPTP containing anti-serum, and immuno-precipitated proteins recovered by Protein A/G were separated on PAGE (12%), and HCPTPA detected using a mouse anti-sheep monoclonal antibody.

Applications for LMW-PTP/ACP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

: 1-10ug/ml

Western Blot

: 1-10ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

Phosphate buffered saline

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMW-PTP/ACP1

The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.

Long Name

Low Molecular Weight Phosphotyrosine Protein Phosphotase

Alternate Names

ACP1, HAAP, LMWPTP

Gene Symbol

ACP1

Additional LMW-PTP/ACP1 Products

Product Documents for LMW-PTP/ACP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for LMW-PTP/ACP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Phosphatases and Regulators

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