MDFI Antibody

Novus Biologicals | Catalog # NBP1-49886

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MDFI Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence YQVSGQRPSGCD corresponding to N-Terminus according to NP_005577.1.

Epitope

YQVSGQRPSGCD - N Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Applications for MDFI Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 20 kDa band in human skeletal muscle lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0 kDa band according to NP_005577.1. The 20 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MDFI

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.

Alternate Names

I-MF, I-mfa, inhibitor of MyoD family a, MyoD family inhibitor, Myogenic repressor I-mf

Entrez Gene IDs

4188 (Human)

Gene Symbol

MDFI

UniProt

NP_005577.1 (Human)

Additional MDFI Products

Product Documents for MDFI Antibody

Certificate of Analysis

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Product Specific Notices for MDFI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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