MDGA-1 (MAM domain-containing GPI anchor protein 1) is a 135-140 kDa, novel member of the Ig-superfamily. It is expressed on embryonic neurons destined for cortical layers 2/3, as well as migrating basilar pontine neurons and D1 interneurons of the spinal cord. Human proMDGA-1 is 937 amino acids (aa) in length (SwissProt #:Q8NFP4). It is a GPI-linked glycoprotein that contains six consecutive Ig-like domains (aa #24-631), followed by one fibronectin type III domain (aa #640-739), a MAM region (aa #751-918), and a cleavable propeptide (aa #933-955). There are at least three potential variants. One shows a 48 aa substitution for aa #936-955, a second shows a 58 aa substitution for aa #847-955, and a third shows a 51 aa substitution for aa #537-955. Over aa #19-931, human MDGA-1 is 95% aa identical to mouse MDGA-1.
MDGA1 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # H00266727-B01P-50ug
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Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Mouse IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
MDGA1 (AAI56660.1, 1 a.a. - 955 a.a.) full-length human protein. MEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIREGDTLMLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPMLTVHQTVSDVRGNFYQEKTVFLRCTVNSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFRLTNTTAPPALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLQWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSMKNATFQITPDVIKESENIQLGQDLKLSCHVDAVPQEKVTYQWFKNGKPARMSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPGAPVPDLSVEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLPLEETPDGKLRLERVSRDMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKVVVMPGSGAPCQSSPQLWGPMAIFLLALQR
Clonality
Polyclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for MDGA1 Antibody - Azide and BSA Free
Western Blot: MDGA1 Antibody [H00266727-B01P-50ug]
Western Blot: MDGA1 Antibody [H00266727-B01P-50ug] - analysis of MDGA1 expression in transfected 293T cell line (H00266727-T01) by MDGA1 MaxPab polyclonal antibody.Lane 1: MDGA1 transfected lysate(105.05 KDa).Lane 2: Non-transfected lysate.Applications for MDGA1 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.4)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MDGA1
Long Name
MAM Domain Containing Glycosylphosphatidylinositol Anchor 1
Alternate Names
GPIM, MAMDC3
Entrez Gene IDs
266727 (Human)
Gene Symbol
MDGA1
OMIM
609626 (Human)
UniProt
Additional MDGA1 Products
Product Documents for MDGA1 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MDGA1 Antibody - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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