MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Cy5]

Name: MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Cy5]
Brand: Novus Biologicals
SKU: NBP1-43070
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-43070

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Product Specifications
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

PE/Cy5 (Excitation = 480;565;650 nm, Emission = 670 nm)

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # M5/114.15.2

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Product Specifications

Immunogen

The immunogen for this antibody was MHC Class II.

Reactivity Notes

Use in Human reported in scientific literature (PMID:29990503).

Specificity

The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Applications for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Cy5]

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Application Notes

The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MHC class II (I-A/I-E)

The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

Long Name

Major Histocompatibility Complex Class II IA and IE

Alternate Names

H2-Ea, MHC class II IA/IE

Gene Symbol

H2-Ea

Additional MHC class II (I-A/I-E) Products

Product Documents for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Cy5]

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Certificate of Analysis

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Product Specific Notices for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Cy5]

Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.