MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free

Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Biotin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with 0.015 ug of Rat IgG2b K Isotype Control Biotin (open histogram) or 0.015 ug of Anti-Mouse MHC Class II (I-A/I-E) Biotin followed by Streptavidin PE (filled histogram).

Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the FITC conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e and 0.06 micrograms of Rat IgG2b k Isotype Control FITC (left) or 0.06 micrograms of Anti-Mouse MHC Class II (I-A/I-E) FITC (right). Cells in the lymphocyte gate were used for analysis.

Western Blot: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312] -

TGF-beta -IRE1 alpha signaling inhibits immunological characteristics of muscle cells by attenuating p38 MAPK pathway. Western blot analyses of the expression changes of H-2Kb, H2-E alpha, or TLR3 in MPC-myotubes derived from Con and SM TGF-beta r2−/− mice (A), or in TGF-beta r2−/− MPC-myotubes (B) with or without stimulation of IFN-gamma /LPS, SRI, 4μ8C, and/or SB (p38 pathway inhibitor). The relative protein expression values are expressed as a ratio (protein of interest/GAPDH). All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312] -

TGF-beta -IRE1 alpha signaling inhibits immunological characteristics of muscle cells by attenuating p38 MAPK pathway. Western blot analyses of the expression changes of H-2Kb, H2-E alpha, or TLR3 in MPC-myotubes derived from Con and SM TGF-beta r2−/− mice (A), or in TGF-beta r2−/− MPC-myotubes (B) with or without stimulation of IFN-gamma /LPS, SRI, 4μ8C, and/or SB (p38 pathway inhibitor). The relative protein expression values are expressed as a ratio (protein of interest/GAPDH). All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312] -

TGF-beta signaling inhibits immunological characteristics of myofibers through activating the IRE1 alpha pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1 alpha and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS and/or SRI (A); 24 or 48 h stimulation of IFN-gamma /LPS, SRI, 4-PBA, 4μ8C (IRE1 alpha pathway inhibitor) or GSK (PERK pathway inhibitor) (B, D). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta, IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312] -

Intrinsic TGF-beta signal controls immunological molecules and myokine expression in cultured primary myotubes. A Representative immunofluorescence staining for desmin, myogenin, eMHC, and TGF-beta r2 in SM TGF-beta r2−/− mice-derived MPC-myotubes (MPCs) that received pro-inflammatory stimuli or not. B, C Western blot analysis of the protein expression of p-Smad2/3, H-2Kb, H2-E alpha, and TLR3 in MPC-myotubes that received pro-inflammatory stimuli or not. D Luminex assay of protein level changes for pro-inflammatory myokines in control or SM TGF-beta r2−/− mice-derived MPC-myotubes exposed to inflammatory milieu or not. Before creating the heat map, log transformation was used to process the data and plot in R language. The relative protein levels are expressed as a ratio (protein of interest/GAPDH). All data are presented as means +/- SD (n = 3 replicates). One-way ANOVA was used for multiple comparisons (**P < 0.01; ***P < 0.001). Bar = 50 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312] -

Intrinsic TGF-beta signaling affects muscle cell immune behaviors by prompting UPR activity under pro-inflammatory conditions. The protein levels of IRE1 alpha and eIF2 alpha (A), H-2Kb, H2-E alpha, and TLR3 (B) in control or SM TGF-beta r2−/− mice-derived MPC-myotubes, with or without stimulation of IFN-gamma /LPS, Tg, or TM, were analyzed by Western blot. The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta, IL-6, and MCP-1 in MPC-myotubes with or without 48 h stimulation of IFN-gamma /LPS, Tg, or TM, analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312] -

TGF-beta signaling inhibits immunological characteristics of myofibers through activating the IRE1 alpha pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1 alpha and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS and/or SRI (A); 24 or 48 h stimulation of IFN-gamma /LPS, SRI, 4-PBA, 4μ8C (IRE1 alpha pathway inhibitor) or GSK (PERK pathway inhibitor) (B, D). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta, IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312] -

TGF-beta signaling inhibits immunological characteristics of myofibers through activating the IRE1 alpha pathway. Western blot analyses of the expression changes of Smad pathway proteins, IRE1 alpha and PERK pathway proteins, and immunological molecules in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS and/or SRI (A); 24 or 48 h stimulation of IFN-gamma /LPS, SRI, 4-PBA, 4μ8C (IRE1 alpha pathway inhibitor) or GSK (PERK pathway inhibitor) (B, D). The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1 beta, IL-6, and MCP-1 in MPC-myotubes isolated from SM TGF-beta r2−/− mice, with or without 48 h stimulation of IFN-gamma /LPS, SRI and/or 4-PBA,analyzed by qPCR. All data are presented as means +/- SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36849929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.