Recombinant Human MICB Protein

Novus Biologicals | Catalog # NBP2-34994

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

ELISA, Affinity Purification, Bioactivity, Microarray, SDS-PAGE
View all MICB Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 287 amino acids corresponding to MICB Source: E. coli

Amino Acid Sequence: AEPHSLRYNL MVLSQDESVQ SGFLAEGHLD GQPFLRYDRQ KRRAKPQGQW AEDVLGAKTW DTETEDLTEN GQDLRRTLTH IKDQKGGLHS LQEIRVCEIH EDSSTRGSRH FYYDGELFLS QNLETQESTV PQSSRAQTLA MNVTNFWKED AMKTKTHYRA MQADCLQKLQ RYLKSGVAIR RTVPPMVNVT CSEVSEGNIT VTCRASSFYP RNITLTWRQD GVSLSHNTQQ WGDVLPDGNG TYQTWVATRI RQGEEQRFTC YMEHSGNHGT HPVPSGKVLV LQSQRTD

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of MICB as determined by LAL method.

Predicted Molecular Mass

32.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

MICB Protein is fully biologically active when compared to standard. The specific activity is determined by binding MICB antibody in ELISA.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-34994
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.0.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MICB

MIC-B (MHC class I chain-related gene B) is a single-pass type I member protein. It is widely expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In addition to this, it is produced by hepatocellular carcinomas, which is only in tumor cells but not in surrounding non-cancerous tissue and can be induced by bacterial and viral infections. MIC-B shares 85 % amino acid identity with MIC-A and they are distantly related to the MHC class I proteins. Because they possess three extracellular Ig-like domains, but unlike classical MHC class I molecules, they do not form a heterodimer with beta2 microglobulin, but bind as a monomer to a KLRK1/NKG2D that is an activating receptor expressed on NK cells, NKT cells, gammadelta T cells, and CD8+ alphabeta T cells. Recognition of MIC-B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MIC-B recognition plays an important role in tumor surveillance, viral infections, and autoimmune diseases.

Long Name

MHC Class I-related Protein B

Alternate Names

MHC class I chain-related protein B, MHC class I mic-B antigen, MHC class I polypeptide-related sequence B, MIC-B, PERB11.2MHC class I-like molecule PERB11.2-IMX, stress inducible class I homolog

Gene Symbol

MICB

Additional MICB Products

Product Documents for Recombinant Human MICB Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Human MICB Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Natural Killer T (NKT) Cells

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