Amino Acid Sequence: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Midkine as determined by LAL method.

Predicted Molecular Mass

13.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Midkine Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Midkine Protein

SDS-PAGE: Recombinant Human Midkine Protein [NBP2-34997]

SDS-Page: Human Midkine Protein [NBP2-34997]

Formulation, Preparation, and Storage

NBP2-34997

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Midkine

Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family. It is encoded by the MDK gene. The Midkine protein includes five intrachain disulfide bonds which hold two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.3 kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Across species, MK shows 87 % identity between the human and murine proteins.

Alternate Names

ARAP, MDK, MEK, MK1, NEGF2

Gene Symbol

MDK

UniProt

P21741

Additional Midkine Products

Product Documents for Recombinant Human Midkine Protein

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Product Specific Notices for Recombinant Human Midkine Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Recombinant Human Midkine Protein

Novus Biologicals | Catalog # NBP2-34997

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