MNI caged kainic acid

Biological Activity

Kainic acid (Cat.No. 0222) caged with the photosensitive 4-methoxy-7-nitroindolinyl group. Generates large inward currents at resting membrane potential upon wide field photolysis in Purkinje neurons. Selectively photoactivates kainate receptors when used in conjunction with an AMPA antagonist, such as GYKI 53655 (Cat.No. 2555). Suitable for uncaging with 300-380 nm excitation. Exhibits rapid uncaging rate relative to ionotropic glutamate receptor activation. Also suitable for two-photon uncaging experiments at 720 nm.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Background References

1. Comparative one- and two-photon uncaging of MNI-glutamate and MNI-kainate on hippocampal CA1 neurons.
   Passlick and Ellis-Davies et al.
   J.Neurosci.Methods., 2017;
2. New caged neurotransmitter analogs selective for glutamate receptor sub-types based on methoxynitroindoline and nitrophenylethoxycarbonyl caging groups.
   Palma-Cerda et al.
   Neuropharmacology., 2012;63:624
3. Comparative analysis of inhibitor effects of caged ligands for the NMDA receptor.
   Maier et al.
   J.Neurosci.Meths., 2005;142:1
4. New caged neurotransmitter analogues based on methoxy-nitroindole and nitrophenylethoxycarbonyl caging groups are selective for glutamate receptor subtypes.
   Palma-Cerda et al.
   Neuropharmacology, 2013;63:624

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