Mouse DMBT1 Alexa Fluor® 350-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5915U-100UG
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Mouse DMBT1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DMBT1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CD163 and rhSRCRB4D is observed.
Source
Monoclonal Rat IgG2b Clone # 548031
Immunogen
E. Coli-derived recombinant mouse DMBT1
Trp215-Gly420
Accession # Q60997
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
CMT‑93 mouse rectal carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DMBT1

DMBT1 (deleted in malignant brain tumors 1), also called salivary agglutinin (SAG) in humans, hensin in rabbit, ebnerin in rat, and muclin, crp-ductin or vomeroglandin in mouse, is a transmembrane or secreted ~340 kDa, 2085 amino acid (aa) protein of the scavenger receptor cysteine-rich (SRCR) family. It contains several SRCR and CUB domains and a zona pellucida motif. The region used as the immunogen (aa 215-420) contains most of SRCR 2 and 3, and shares ~74% aa identity with corresponding regions of rat or human DMBT1. Several alternately spliced, glycosylated and sulfated forms exist. DMBT1 functions in mucosal immunity by binding bacterial, viral and endogenous proteins to aid clearance by macrophages. It is also thought to play a role in epithelial cell differentiation and tumor suppression.

Long Name
Deleted in Malignant Brain Tumors 1
Entrez Gene IDs
1755 (Human); 12945 (Mouse); 170568 (Rat)
Alternate Names
CRP-ductin; deleted in malignant brain tumors 1 protein; deleted in malignant brain tumors 1; DMBT1; Ebnerin; FLJ61058; Glycoprotein 340; GP340; gp-340; GP340muclin; Hensin; MGC164738; Muclin; SAG; Salivary agglutinin; Surfactant pulmonary-associated D-binding protein; Vomeroglandin

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