Mouse DMBT1 Antibody

Catalog # Availability Size / Price Qty
MAB5915
MAB5915-SP
Detection of DMBT1 in CMT‑93 Mouse Cell Line by Flow Cytometry.
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Mouse DMBT1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DMBT1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CD163 and rhSRCRB4D is observed.
Source
Monoclonal Rat IgG2B Clone # 548031
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. Coli-derived recombinant mouse DMBT1
Trp215-Gly420
Accession # Q60997
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of DMBT1 in CMT‑93 Mouse Cell Line by Flow Cytometry. View Larger

Detection of DMBT1 in CMT‑93 Mouse Cell Line by Flow Cytometry. CMT‑93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse DMBT1 Monoclonal Antibody (Catalog # MAB5915, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DMBT1

DMBT1 (deleted in malignant brain tumors 1), also called salivary agglutinin (SAG) in humans, hensin in rabbit, ebnerin in rat, and muclin, crp-ductin or vomeroglandin in mouse, is a transmembrane or secreted ~340 kDa, 2085 amino acid (aa) protein of the scavenger receptor cysteine-rich (SRCR) family. It contains several SRCR and CUB domains and a zona pellucida motif. The region used as the immunogen (aa 215-420) contains most of SRCR 2 and 3, and shares ~74% aa identity with corresponding regions of rat or human DMBT1. Several alternately spliced, glycosylated and sulfated forms exist. DMBT1 functions in mucosal immunity by binding bacterial, viral and endogenous proteins to aid clearance by macrophages. It is also thought to play a role in epithelial cell differentiation and tumor suppression.

Long Name
Deleted in Malignant Brain Tumors 1
Entrez Gene IDs
1755 (Human); 12945 (Mouse); 170568 (Rat)
Alternate Names
CRP-ductin; deleted in malignant brain tumors 1 protein; deleted in malignant brain tumors 1; DMBT1; Ebnerin; FLJ61058; Glycoprotein 340; GP340; gp-340; GP340muclin; Hensin; MGC164738; Muclin; SAG; Salivary agglutinin; Surfactant pulmonary-associated D-binding protein; Vomeroglandin

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