Mouse Patched 1/PTCH (First Extracellular Loop) Antibody

Catalog # Availability Size / Price Qty
Detection of Patched 1/PTCH in Neuro‑2A Mouse Cell Line by Flow Cytometry.
1 Image
Product Details
Citations (1)
Supplemental Products
Reviews (1)

Mouse Patched 1/PTCH (First Extracellular Loop) Antibody Summary

Species Reactivity
Detects mouse Patched 1/PTCH in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Patched 1 is observed, and less than 5% cross-reactivity with recombinant mouse Patched-2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse Patched 1/PTCH
Accession # Q61115
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Patched (First Extracellular Loop)
Flow Cytometry
2.5 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Patched 1/PTCH antibody in Neuro-2A Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Patched 1/PTCH in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Goat Anti-Mouse Patched 1/PTCH (First Extracellular Loop) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4105, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Patched 1/PTCH

Patched (Ptch1) is a 170‑175 kDa protein that functions in Hedgehog signaling. It is found on multiple cell types and forms a constitutive, inactivating complex with Smoothened. Upon Hedgehog binding to Patched, Smoothened is released and initiates downstream signaling. Mouse Patched is a 12 transmembrane, 1434 amino acid (aa) glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for Ptch1 has been reported in mice. However, two or more splice forms may exist, with variation occurring over the first 150 aa of the N-terminus. Mouse Patched shares 97% and 99% aa sequence identity with human and rat Patched, respectively.

Entrez Gene IDs
5727 (Human); 19206 (Mouse); 89830 (Rat)
Alternate Names
BCNS; BCNSFLJ42602; FLJ26746; HPE7; mes; NBCCS; patched (Drosophila) homolog; Patched 1; patched homolog (Drosophila); patched homolog 1 (Drosophila); patched homolog 1; protein patched homolog 1; PTC; ptc1; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH; PTCH1; PTCH11

Product Datasheets

You must select a language.


Citation for Mouse Patched 1/PTCH (First Extracellular Loop) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Toll-like receptor 3 regulates neural stem cell proliferation by modulating the Sonic Hedgehog pathway.
    Authors: Yaddanapudi K, De Miranda J, Hornig M
    PLoS ONE, 2011-10-25;6(10):e26766.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Patched 1/PTCH (First Extracellular Loop) Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Mouse Patched 1/PTCH (First Extracellular Loop) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Mouse Patched 1/PTCH (First Extracellular Loop) Antibody
By Alina Filatova on 01/23/2019
Application: Flow Sample Tested: Differentiated iPS cells Species: Human