Mouse Patched 1/PTCH (First Extracellular Loop) Antibody Summary
Accession # Q61115
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Patched 1/PTCH in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Goat Anti-Mouse Patched 1/PTCH (First Extracellular Loop) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4105, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Patched 1/PTCH
Patched (Ptch1) is a 170‑175 kDa protein that functions in Hedgehog signaling. It is found on multiple cell types and forms a constitutive, inactivating complex with Smoothened. Upon Hedgehog binding to Patched, Smoothened is released and initiates downstream signaling. Mouse Patched is a 12 transmembrane, 1434 amino acid (aa) glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for Ptch1 has been reported in mice. However, two or more splice forms may exist, with variation occurring over the first 150 aa of the N-terminus. Mouse Patched shares 97% and 99% aa sequence identity with human and rat Patched, respectively.
Citation for Mouse Patched 1/PTCH (First Extracellular Loop) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Toll-like receptor 3 regulates neural stem cell proliferation by modulating the Sonic Hedgehog pathway.
Authors: Yaddanapudi K, De Miranda J, Hornig M
PLoS ONE, 2011;6(10):e26766.
Sample Types: Whole Cells
Applications: Flow Cytometry
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