Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

Catalog # Availability Size / Price Qty
VC002-025
VC002-050
VC002-125
VC002-250
Detection of FABP1/L-FABP in Human Kidney.
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Product Details
Citations (1)
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody Summary

Species Reactivity
Mouse, Rabbit
Specificity
Detects heavy ( gamma ) chains on mouse and rabbit IgG and light chains on all mouse and rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin mouse or rabbit serum proteins or serum proteins from bovine, horse, human, pig or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse and Rabbit IgG
Formulation
Supplied as a solution in PBS with preservative.
Label
HRP Polymer

Applications

Recommended Concentration
Sample
Immunohistochemistry
Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Detection of FABP1/L-FABP in Human Kidney. View Larger

Detection of FABP1/L-FABP in Human Kidney. FABP1/L-FABP was detected in paraffin-embedded sections of human kidney using Mouse Anti-Human FABP1/L-FABP Monoclonal Antibody (Catalog # MAB2964) at 1 μg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC002). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

Immunohistochemistry Detection of Phospho-p38 (T180/Y182) in Mouse Embryo (13 d.p.c.). View Larger

Detection of Phospho-p38 (T180/Y182) in Mouse Embryo (13 d.p.c.). Phospho-p38 (T180/Y182) was detected in frozen sections of mouse embryo (13 d.p.c., cross-section through the gut) using Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869) at 1 μg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC002). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citation for Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Th1/Th2 PB balance and CD200 expression of patients with active severe alopecia areata
    Authors: X Ma, S Chen, W Jin, Y Gao
    Exp Ther Med, 2017;13(6):2883-2887.
    Species: Human
    Sample Types: Whole Tissue

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