Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
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  • Species Reactivity
    Mouse, Rabbit
  • Specificity
    Detects heavy ( gamma ) chains on mouse and rabbit IgG and light chains on all mouse and rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin mouse or rabbit serum proteins or serum proteins from bovine, horse, human, pig or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse and Rabbit IgG
  • Formulation
    Supplied as a solution in PBS with preservative.
  • Label
    HRP Polymer
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Detection of FABP1/L-FABP in Human Kidney. FABP1/L-FABP was detected in paraffin-embedded sections of human kidney using Mouse Anti-Human FABP1/L-FABP Monoclonal Antibody (Catalog # MAB2964) at 1 μg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC002). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).
Immunohistochemistry
Detection of Phospho-p38 (T180/Y182) in Mouse Embryo (13 d.p.c.). Phospho-p38 (T180/Y182) was detected in frozen sections of mouse embryo (13 d.p.c., cross-section through the gut) using Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869) at 1 μg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC002). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).
Preparation and Storage
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.
  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Th1/Th2 PB balance and CD200 expression of patients with active severe alopecia areata
    Authors: X Ma, S Chen, W Jin, Y Gao
    Exp Ther Med, 2017;13(6):2883-2887.
    Species: Human
    Sample Type: Whole Tissue

FAQs

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Secondary Antibodies
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Rabbit IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse IgG HRP-conjugated Antibody

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Mouse IgG Antibody

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Rabbit IgG PE-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL005 2
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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