Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody

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PPS027
Detection of GABAAR  alpha 5 C-Terminus by Western Blot.
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Citations (1)
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Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Mouse and rat ~55 kDa GABAA R alpha 5 subunit, C-Terminus
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Fusion protein from the cytosolic loop of the rat GABAA R alpha 5 subunit, C-Terminus
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of GABA<sub>A</sub>R a5 C-Terminus antibody by Western Blot. View Larger

Detection of GABAAR alpha 5 C-Terminus by Western Blot. Western blot of 7 μg of rat hippocampus showing specific immunolabeling of the ~55 kDa alpha 5 subunit of the GABAAR.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 5

GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha 5 isoform is a 439 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 25 - 232). The alpha 5 subunit is unusual in that it contains an N-terminal histidine which renders it very sensitive to zinc. It is also associated with extrasynaptic sites and seems to mediate tonic inhibition in hippocampal neurons.

References
  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Caraiscos, V.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:3662.
  4. Fisher, J.L. (2002) Neuropharmacology 42:922.
  5. Loebrich, S. et al. (2006) EMBO J. 25:987.
Long Name
gamma-Aminobutyric Acid Type A Receptor, alpha-5 Polypeptide
Entrez Gene IDs
2558 (Human); 110886 (Mouse); 29707 (Rat)
Alternate Names
GABA A R alpha 5; GABA(A) receptor subunit alpha-5; GABAAR alpha 5; GABAARa5; GABRA5; gamma-aminobutyric acid (GABA) A receptor, alpha 5; gamma-aminobutyric acid receptor subunit alpha-5; MGC138184

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Citation for Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. GL-II-73, a Positive Allosteric Modulator of alpha 5GABAA Receptors, Reverses Dopamine System Dysfunction Associated with Pilocarpine-Induced Temporal Lobe Epilepsy
    Authors: McCoy, AM;Prevot, TD;Sharmin, D;Cook, JM;Sibille, EL;Lodge, DJ;
    International journal of molecular sciences
    Species: Rat
    Sample Types: Protein
    Applications: Western Blot

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