Mouse Semaphorin 4C PE‑conjugated Antibody

R&D Systems | Catalog # FAB8497P

R&D Systems
Discontinued Product
FAB8497P has been discontinued. View all Semaphorin 4C products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 663203
View all Semaphorin 4C Primary Antibodies »

Product Specifications

Immunogen

Mouse myelona cell line NS0-derived recombinant mouse Semaphorin 4C
Glu22-Gly664
Accession # Q64151

Specificity

Detects HEK293 human embryonic cell line transfected with mouse Semaphorin 4C by flow cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Semaphorin 4C PE‑conjugated Antibody

Detection of Semaphorin 4C antibody in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4C and eGFP antibody by Flow Cytometry.

Detection of Semaphorin 4C in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4C and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4C and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4C PE-conjugated Monoclonal Antibody (Catalog # FAB8497P) or (B) Rat IgG1Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Semaphorin 4C PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4C and eGFP

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 4C

Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature mouse Sema4C is a type I transmembrane glycoprotein that is 814 amino acids (aa) in length. It contains a 644 aa extracellular region (aa 21-664) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑552), and an Ig-like C2-type domain (aa 557-645). The cytoplasmic region interacts with PZD-domain containing proteins. Over aa 21-664, mouse Sema4C shares 85% and 95% aa identity with human and rat Sema4C, respectively.

Alternate Names

SEMA4C, SEMACL1, Semaf, SEMAI

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Gene Symbol

SEMA4C

UniProt

Q64151

Additional Semaphorin 4C Products

Product Documents for Mouse Semaphorin 4C PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Semaphorin 4C PE‑conjugated Antibody

For research use only

Related Research Areas

Proteoglycans

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