NDC80 Antibody (JE31-10)

Novus Biologicals | Catalog # NBP3-32419

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE31-10 expressed in HEK293
View all NDC80 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human NDC80 aa 593-642 / 642. (Uniprot: O14777)

Localization

Nucleus, Chromosome, centromere, kinetochore.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDC80 Antibody (JE31-10)

NDC80 Antibody (JE31-10)

Western Blot: NDC80 Antibody (JE31-10) [NBP3-32419]

Western Blot: NDC80 Antibody (JE31-10) [NBP3-32419] - Western blot analysis of NDC80 on different lysates with Rabbit anti-NDC80 antibody (NBP3-32419) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: HeLa cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: HepG2 cell lysate
Lane 8: LO2 cell lysate

Lysates/proteins at 15 ug/Lane.

Predicted band size: 74 kDa
Observed band size: 74 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32419) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for NDC80 Antibody (JE31-10)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDC80

Human HEC1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results in the impairment of chromosome congression and results in spindle checkpoint activation.

Alternate Names

HEC1KNTC2, HECRetinoblastoma-associated protein HEC, Highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, highly expressed in cancer, rich in leucine heptad repeats (yeast), HsHec1, hsNDC80, kinetochore associated 2, Kinetochore protein Hec1, kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3

Gene Symbol

NDC80

Additional NDC80 Products

Product Documents for NDC80 Antibody (JE31-10)

Certificate of Analysis

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Product Specific Notices for NDC80 Antibody (JE31-10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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