NEDD4 Antibody

Novus Biologicals | Catalog # NBP2-27201

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Equine, Primate

Predicted:

Monkey (100%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

A portion of amino acids 200-260 of human NEDD4 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 88% homologous in Chicken and Opossum.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit NEDD4 Antibody (NBP2-27201) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NEDD4 Antibody

Western Blot: NEDD4 Antibody [NBP2-27201]

Western Blot: NEDD4 Antibody [NBP2-27201]

Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for NEDD4 Antibody

Application
Recommended Usage

Western Blot

4-6ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEDD4

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/kDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.

Long Name

Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4

Alternate Names

RPF1

Gene Symbol

NEDD4

UniProt

P46934 (Human)

Additional NEDD4 Products

Product Documents for NEDD4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NEDD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases
Notch Pathway

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