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Key Product Details
Species
Mouse
Applications
SDS-PAGE
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Product Specifications
Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.
Type
Cell
Formulation, Preparation, and Storage
Formulation
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added.
Concentration
2.5 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C in the dark. Avoid freeze-thaw cycles.
Background: NIH 3T3
Additional NIH 3T3 Products
Product Documents for NIH 3T3 Cell Lysate
Product Specific Notices for NIH 3T3 Cell Lysate
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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