Nogo-A, also known as Reticulon 4 (RTN4) is a transmembrane protein that is mainly expressed in oligodendrocytes of the central nervous system but is also reported in fibroblasts, dorsal root ganglion neurons, macrophages, and myoblasts. Binding of the Nogo66 region to the Nogo Receptor/p75 complex on axons induces growth cone collapse and blocks neurite outgrowth. Nogo-A interactions with the Nogo-B receptor (NgBR/NUS1) can regulate vascular remodeling, inhibit neuronal differentiation, and promote glial formation from neural progenitors.
Nogo-A Antibody (391401) [mFluor Violet 450 SE]
Novus Biologicals | Catalog # FAB30981MFV450
Key Product Details
Species Reactivity
Rat
Applications
Western Blot
Label
mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 391401
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Product Specifications
Immunogen
E. coli-derived recombinant rat Nogo-A
Glu2-Val172
Accession # Q9JK11
Glu2-Val172
Accession # Q9JK11
Specificity
Detects rat Nogo-A in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Nogo-A Antibody (391401) [mFluor Violet 450 SE]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Nogo-A
Long Name
Reticulon 4A
Alternate Names
NI220, NogoA, RTN4, RTN4A
Gene Symbol
RTN4
Additional Nogo-A Products
Product Documents for Nogo-A Antibody (391401) [mFluor Violet 450 SE]
Product Specific Notices for Nogo-A Antibody (391401) [mFluor Violet 450 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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