Nucleolin [p Thr76] Antibody (24GB145)

Novus Biologicals | Catalog # NBP3-50509

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB145
View all Nucleolin Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-Nucleolin (T76) (Uniprot: P19338)

Modification

p Thr76

Specificity

This antibody detects endogenous levels of NCL only when phosphorylated at T76

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nucleolin [p Thr76] Antibody (24GB145)

Nucleolin [p Thr76] Antibody (24GB145)

Western Blot: Nucleolin [p Thr76] Antibody (24GB145)[NBP3-50509] -

Western blotting analysis using Nucleolin (T76) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Nucleolin [p Thr76] Antibody (24GB145)

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nucleolin

Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. Nucleolin antibodies are commonly used as a mammalian nucleolus marker.

Alternate Names

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Gene Symbol

NCL

Additional Nucleolin Products

Product Documents for Nucleolin [p Thr76] Antibody (24GB145)

Certificate of Analysis

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Product Specific Notices for Nucleolin [p Thr76] Antibody (24GB145)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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