Nuf2 Antibody (S07-9E2)

Novus Biologicals | Catalog # NBP3-19724

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-9E2 expressed in HEK293
View all Nuf2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Nuf2 (Uniprot # Q9BZD4)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nuf2 Antibody (S07-9E2)

Western Blot: Nuf2 Antibody (S07-9E2) [NBP3-19724]

Western Blot: Nuf2 Antibody (S07-9E2) [NBP3-19724]

Western Blot: Nuf2 Antibody (S07-9E2) [NBP3-19724] - Western blot detection of Nuf2 in K562 cell lysates using NBP3-19724 (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 54kDa.

Applications for Nuf2 Antibody (S07-9E2)

Application
Recommended Usage

Western Blot

1/1000-1/5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nuf2

Nuf2, also known as Kinetochore protein Nuf2, is a 54 kDa 464 amino acid protein, and it is involved in chromosome segregation, specifically as it relates to spindle checkpoint activity with the kinetochore-associated NDC80 complex. This protein is necessary for kinetochore stability and organization of microtubule binding sites on the outer plate, forming interactions with NDC80/HEC-1-CDCA1, SPBC24-SPBC25, Bub1, EXOC1, as well as AURKB/Aurora-B and CENPE in the mitotic prometaphase, DNA replication, and Cell Cycle pathways. Current research is being performed on this proteins involvement in gastric, lung, and breast cancers, in addition to essential hypertension, schizophrenia, and duodenogastric reflux.

Alternate Names

cancer/testis antigen 106, CDCA1hsNuf2, cell division cycle associated 1, Cell division cycle-associated protein 1, CT106, hNuf2, kinetochore protein Nuf2, NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae), NUF2RhNuf2R

Gene Symbol

NUF2

Additional Nuf2 Products

Product Documents for Nuf2 Antibody (S07-9E2)

Certificate of Analysis

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Product Specific Notices for Nuf2 Antibody (S07-9E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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