p38 alpha/beta/gamma Antibody (PSH03-60)

Western Blot: p38 alpha/beta/gamma Antibody (PSH03-60) [NBP3-33147]

Western Blot: p38 alpha/beta/gamma Antibody (PSH03-60) [NBP3-33147] - Western blot analysis of p38 alpha/beta/gamma on different lysates with Rabbit anti-p38 alpha/beta/gamma antibody (NBP3-33147) at 1/2,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: Neuro-2a cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse skeletal muscle tissue lysate
Lane 9: Rat skeletal muscle tissue lysate
Lane 10: Rat heart tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.