Catalog Number: 4192
Alternate Names: PD325901
Chemical Name: N-[(2R)-2,3-Dihydroxypropoxy]-3,4-difluoro-2-[(2-fluoro-4-iodophenyl)amino]-benzamide
Biological Activity
Potent MEK1 and MEK2 inhibitor. Inhibits MEK activity in mouse colon 26 cells (IC50 = 0.33 nM). Inhibits the growth of melanoma cell lines in vitro and in vivo; induces G1-phase cell cycle arrest and apoptosis in a mouse xenograft model. Also inhibits production of proangiogenic cytokines such as VEGF. Enhances generation of induced pluripotent stem cells (iPSCs). Orally active.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 25 mM in DMSO
  • Purity:
  • Storage:
    Store at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Additional Information
Licensing Caveats:
Sold for research purposes under agreement from Pfizer Inc.
Background References
  1. Growth-inhibitor and antiangiogenic activity of the MEK inhibitor PD0325901 in malignant melanoma with or without BRAF mutations.
    Ciuffreda et al.
    Neoplasia, 2009;11:720
  2. A chemical platform for improved induction of human iPSCs.
    Lin et al.
    Nat.Methods., 2009;6:805
  3. The discovery of the benzhydroxamate MEK inhibitors CI-1040 and PD 0325901.
    Barrett et al.
    Bioorg.Med.Chem.Lett., 2008;18:6501
  4. The biological profile of PD 0325901: a second generation analog of CI-1040 with improved pharmaceutical potential.
    Sebolt-Leopold et al.
    Proc.Amer.Assoc.Cancer Res., 2004;45:925

The citations listed below are publications that use Tocris products. Selected citations for PD 0325901 include:

Showing Results 1 - 9 of 9

  1. Poly(I:C) increases the expression of mPGES-1 and COX-2 in rat primary microglia.
    Authors: Oliveira Et al.
    Stem Cells
  2. Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors.
    Authors: Maza Et al.
    Nat Biotechnol
  3. HD iPSC-derived neural progenitors accumulate in culture and are susceptible to BDNF withdrawal due to glutamate toxicity.
    Authors: Mattis Et al.
    PLoS One
  4. Functional annotation of native enhancers with a Cas9-histone demethylase fusion.
    Authors: Kearns Et al.
    Nat Methods
  5. The bimodally expressed microRNA miR-142 gates�exit from pluripotency.
    Authors: Sladitschek and Neveu
    J Neuroinflammation
  6. Manganese Superoxide Dismutase Gene Expression Is Induced by Nanog and Oct4, Essential Pluripotent Stem Cells' Transcription Factors.
    Authors: Solari Et al.
    Mol Syst Biol
  7. A method to identify and isolate pluripotent human stem cells and mouse epiblast stem cells using lipid body-associated retinyl ester fluorescence.
    Authors: Muthusamy Et al.
    Stem Cell Reports
  8. Glycogen synthase kinase 3β and activin/nodal inhibition in human embryonic stem cells induces a pre-neuroepithelial state that is required for specification to a floor plate cell lineage.
    Authors: Denham Et al.
  9. Conversion from mouse embryonic to extra-embryonic endoderm stem cells reveals distinct differentiation capacities of pluripotent stem cell states.
    Authors: Cho Et al.
    Cancer Biol Ther
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.

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