Chemical Name: N-[(2R)-2,3-Dihydroxypropoxy]-3,4-difluoro-2-[(2-fluoro-4-iodophenyl)amino]-benzamide
Biological ActivityPD 0325901 is a potent MEK1 and MEK2 inhibitor. Inhibits MEK activity in mouse colon 26 cells (IC50 = 0.33 nM). Inhibits the growth of melanoma cell lines in vitro and in vivo; induces G1-phase cell cycle arrest and apoptosis in a mouse xenograft model. Also inhibits production of proangiogenic cytokines such as VEGF. Enhances generation of induced pluripotent stem cells (iPSCs). Orally active.
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3D mouse embryonic stem cell culture for generating inner ear organoids.
Koehler KR, Hashino E.
A chemical platform for improved induction of human iPSCs.
Lin et al.
The discovery of the benzhydroxamate MEK inhibitors CI-1040 and PD 0325901.
Barrett et al.
Growth-inhibitor and antiangiogenic activity of the MEK inhibitor PD0325901 in malignant melanoma with or without BRAF mutations.
Ciuffreda et al.
The biological profile of PD 0325901: a second generation analog of CI-1040 with improved pharmaceutical potential.
Sebolt-Leopold et al.
Proc.Amer.Assoc.Cancer Res., 2004;45:925
Citations for PD 0325901
The citations listed below are publications that use Tocris products. Selected citations for PD 0325901 include:
21 Citations: Showing 1 - 10
A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos.
Authors: Canizo Et al.
BMC Dev Biol 2019;19:13
Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells.
Authors: Zhang Et al.
Nat Commun 2019;10:496
Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells.
Nat Commun 2018;9(1):475
Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector.
Nat Med 2018;24(5):647
CRISPR-based chromatin remodeling of the endogenous Oct4 or Sox2 locus enables reprogramming to pluripotency.
Authors: Liu Et al.
Cell Stem Cell. 2018;22:252
Distinctive features of lincRNA gene expression suggest widespread RNA-independent functions.
Authors: Tuck Et al.
Life Sci Alliance 2018;1:e201800124
PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation.
Authors: Gupta Et al.
Mol Cell 2017;65:999
Gene Resistance to Transcriptional Reprogramming following Nuclear Transfer Is Directly Mediated by Multiple Chromatin-Repressive Pathways.
Authors: Jullien Et al.
Mol Cell 2017;65:873
Stem Cell Differentiation as a Non-Markov Stochastic Process.
Authors: Stumpf Et al.
Cell Syst 2017;5:268
Establishment of mouse expanded potential stem cells.
Authors: Yang Et al.
The intrinsically kinase-inactive EPHB6 receptor predisposes cancer cells to DR5-induced apoptosis by promoting mitochondrial fragmentation.
Authors: Zawily Et al.
Poly(I:C) increases the expression of mPGES-1 and COX-2 in rat primary microglia.
Authors: Oliveira Et al.
Stem Cells 2016;13:11
Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors.
Authors: Maza Et al.
Nat Biotechnol 2015;33:769
HD iPSC-derived neural progenitors accumulate in culture and are susceptible to BDNF withdrawal due to glutamate toxicity.
Authors: Mattis Et al.
PLoS One 2015;24:3257
Effects of AKT inhibition on HGF-mediated erlo. resistance in non-small cell lung cancer cell lines.
Authors: Holland Et al.
J Cancer Res Clin Oncol 2015;141:615
Functional annotation of native enhancers with a Cas9-histone demethylase fusion.
Authors: Kearns Et al.
Nat Methods 2015;12:401
The bimodally expressed microRNA miR-142 gates exit from pluripotency.
Authors: Sladitschek and Neveu
J Neuroinflammation 2015;11:850
Manganese Superoxide Dismutase Gene Expression Is Induced by Nanog and Oct4, Essential Pluripotent Stem Cells' Transcription Factors.
Authors: Solari Et al.
Mol Syst Biol 2015;10:e0144336
A method to identify and isolate pluripotent human stem cells and mouse epiblast stem cells using lipid body-associated retinyl ester fluorescence.
Authors: Muthusamy Et al.
Stem Cell Reports 2014;3:169
Glycogen synthase kinase 3β and activin/nodal inhibition in human embryonic stem cells induces a pre-neuroepithelial state that is required for specification to a floor plate cell lineage.
Authors: Denham Et al.
Conversion from mouse embryonic to extra-embryonic endoderm stem cells reveals distinct differentiation capacities of pluripotent stem cell states.
Authors: Cho Et al.
Cancer Biol Ther 2012;139:2866
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All iPSC lines and R1 mouse ES cells were maintained on feeders in KO-DMEM (Invitrogen) with 3 mM CHIR99021, and 1 mM PD0325901.
Used to grow mES cells in 2i conidtions. The cells grew well and I did QPCR on isolated RNA
Mouse ES cells in 2i remain to be in the naive pluripotent state while cells in -2i for 48 hours exit naive pluripotency
Primary lung endothelial cells were pretreated with various concentrations of PD 0325901 for 30 minutes prior to the treatment of IL1b (1ng/ml).