Peroxidase Antibody

Novus Biologicals | Catalog # NBP3-50157

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Peroxidase Primary Antibodies »

Product Specifications

Immunogen

Peroxidase [Horseradish]

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Description

Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against stated buffer.

Applications for Peroxidase Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody has been tested by western blot and is suitable for dot blot, ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Peroxidase

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

Alternate Names

EC 1.11.1.7, Horseradish Peroxidase, HPRC 1, HPRC1, HRPA2, HRPC 2, HRPC2, HRPE5, HRPN, Neutral peroxidase, Peroxidase A2, Peroxidase C1A, Peroxidase C1B, Peroxidase C2, Peroxidase C3, Peroxidase E5, Peroxidase N, PRXC1A, PRXC1B, PRXC2, PRXC3

Additional Peroxidase Products

Product Documents for Peroxidase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Peroxidase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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