Piccolo Antibody (PCLO-01) - BSA Free

Novus Biologicals | Catalog # NBP2-62209

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Flow (Intracellular)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCLO-01

Format

BSA Free
View all Piccolo Primary Antibodies »

Product Specifications

Immunogen

Human recombinant PCLO protein

Specificity

The antibody A53-B/A2 reacts with Rod domain of cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Piccolo Antibody (PCLO-01) - BSA Free

Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209]

Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209]

Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209] - Human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.
Piccolo Antibody (PCLO-01) Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209]

Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209]

Staining of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.

Applications for Piccolo Antibody (PCLO-01) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-5 ug/ml

Flow Cytometry

1-5 ug/ml

Western Blot

1:100-1:2000
Application Notes
A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAGE.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Piccolo

Piccolo is a presynaptic cytomatrix protein concentrated at the presynaptic side of synaptic junctions. Piccolo is a large protein which constists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. It is usually found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1. Piccolo is highly expressed in brain with low levels found in stomach. It is not detected in other tissues analyzed including adrenal gland, testis and pancreas.

Long Name

Presynaptic Cytomatrix Protein

Alternate Names

ACZ, Aczonin, PCLO

Gene Symbol

PCLO

Additional Piccolo Products

Product Documents for Piccolo Antibody (PCLO-01) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Piccolo Antibody (PCLO-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Piccolo Antibody (PCLO-01) - BSA Free

There are currently no reviews for this product. Be the first to review Piccolo Antibody (PCLO-01) - BSA Free and earn rewards!

Have you used Piccolo Antibody (PCLO-01) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies