PIT 1

Tocris Bioscience | Catalog # 4168

PIP3 antagonist. Blocks PIP3-PH interaction; inhibits Akt signaling
Tocris Bioscience
Discontinued Product
4168 has been discontinued. View all Inositol Lipid Antagonists products.

Key Product Details

Description

PIP3 antagonist. Blocks PIP3-PH interaction; inhibits Akt signaling
View all Inositol Lipid Antagonists »

Product Description

PIT 1 is a selective PIP3 antagonist. Blocks the binding of PIP3 to the pleckstrin homology (PH) domain of Akt (IC50 = 31.03 μM). Inhibits cancer cell survival and induces apoptosis by inhibition of PIP3-dependent PI 3-kinase/Akt signaling. Exhibits antitumor activity in vivo.

Product Specifications for PIT 1

Molecular Weight

351.76

Formula

C14H10ClN3O4S

Storage

Store at +4°C

Purity

≥99% (HPLC)

Chemical Name

N-[[(3-Chloro-2-hydroxy-5-nitrophenyl)amino]thioxomethyl]benzamide

CAS Number

53501-41-0

PubChem ID

3664359

InChI Key

RIGXBXPAOGDDIG-UHFFFAOYSA-N

SMILES

ClC1=C(O)C(NC(NC(C2=CC=CC=C2)=O)=S)=CC([N+]([O-])=O)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 26.38 75

Preparing Stock Solutions for PIT 1

The following data is based on the product molecular weight 351.76.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 3.79 mL 18.95 mL 37.90 mL
3.75 mM 0.76 mL 3.79 mL 7.58 mL
7.5 mM 0.38 mL 1.90 mL 3.79 mL
37.5 mM 0.08 mL 0.38 mL 0.76 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 16 publications citing the usage of this product.

  • Miao Small molecule inhibition of phosphatidylinositol-3,4,5-triphosphate (PIP3) binding to pleckstrin homology domains. Proc.Natl.Acad.Sci.

Product Documents for PIT 1

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PIT 1

For research use only

Related Research Areas

Ion Channels and Regulators

Customer Reviews for PIT 1 (1)

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  • Works well
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HCC1937
    Verified Customer | Posted 05/05/2020
    Induction of PAR and gamma H2ax by the PIP3-mimetic PIT1 (0-100uM)
    PIT 1 4168

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