POLR3K Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94027

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POLR3K Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human POLR3K (NP_057394.3). MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR3K Antibody - BSA Free

Western Blot: POLR3K AntibodyBSA Free [NBP2-94027]

Western Blot: POLR3K AntibodyBSA Free [NBP2-94027]

Western Blot: POLR3K Antibody [NBP2-94027] - Analysis of extracts of various cell lines, using POLR3K at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
POLR3K Antibody - BSA Free

Western Blot: Rabbit Polyclonal POLR3K Antibody [NBP2-94027] -

Western Blot: Rabbit Polyclonal POLR3K Antibody [NBP2-94027] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. POLR3K Antibody (NBP2-94027) was used for primary antibody: 1:500, 4℃, overnight. Image from a verified customer review.

Applications for POLR3K Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-94027 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLR3K

POLR3K encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription.

Alternate Names

C11, DNA-directed RNA polymerase III subunit K, DNA-directed RNA polymerase III subunit RPC10, DNA-directed RNA polymerases III 12.5 kDa polypeptide, hRPC11, HsC11p, polymerase (RNA) III (DNA directed) polypeptide K (12.3 kDa), polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa, RNA polymerase III 12.5 kDa subunit, RNA polymerase III subunit (hRPC11), RNA polymerase III subunit C10, RNA polymerase III subunit C11, RNA polymerase III subunit CII, RPC10, RPC11C11-RNP3, RPC12.5

Gene Symbol

POLR3K

Additional POLR3K Products

Product Documents for POLR3K Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for POLR3K Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for POLR3K Antibody - BSA Free (1)

5 out of 5
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  • POLR3K Antibody - BSA Free
    Name: Yongkang Yang
    Application: Western Blot
    Verified Customer | Posted 12/20/2023
    Western Blot: whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. POLR3K Antibody (NBP2-94027) was used for primary antibody: 1:500, 4℃, overnight.
    POLR3K Antibody - BSA Free NBP2-94027

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