PUS3 Overexpression Lysate

Name: PUS3 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-06135
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-06135

Submit a review

Product Documents

100% Guarantee

Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for PUS3 Overexpression Lysate

PUS3 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC201648

Accession#: NM_031307

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.

Theoretical MW

55.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for PUS3 Overexpression Lysate

Western Blot: PUS3 Overexpression Lysate [NBP2-06135]

Western Blot: PUS3 Overexpression Lysate (Adult Normal) [NBP2-06135] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PUS3.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: PUS3

Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs

Alternate Names

EC 5.4.99.-, FKSG32, FLJ23638, pseudouridylate synthase 3,2610020J05Rik, tRNA pseudouridine synthase 3, tRNA pseudouridylate synthase 3, tRNA-uridine isomerase 3

Gene Symbol

PUS3

Additional PUS3 Products

Product Documents for PUS3 Overexpression Lysate

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PUS3 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customer Reviews for PUS3 Overexpression Lysate

There are currently no reviews for this product. Be the first to review PUS3 Overexpression Lysate and earn rewards!

Have you used PUS3 Overexpression Lysate?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image