RAB3IP Antibody - Azide and BSA Free
Novus Biologicals | Catalog # H00117177-B01P
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Key Product Details
Species Reactivity
Validated:
Human, Rat, Zebrafish
Cited:
Fish - Danio rerio (Zebrafish)
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Mouse IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
RAB3IP (NP_071901.2, 1 a.a. - 460 a.a.) full-length human protein. MANDPLEGFHEVNLASPTSPDLLGVYESGTQEQTTSPSVIYRPHPSALSSVPIQANALDVSELPTQPVYSSPRRLNCAEISSISFHVTDPAPCSTSGVTAGLTKLTTRKDNYNAEREFLQGATITEACDGSDDIFGLSTDSLSRLRSPSVLEVREKGYERLKEELAKAQRELKLKDEECERLSKVRDQLGQELEELTASLFEEAHKMVREANIKQATAEKQLKEAQGKIDVLQAEVAALKTLVLSSSPTSPTQEPLPGGKTPFKKGHTRNKSTSSAMSGSHQDLSVIQPIVKDCKEADLSLYNEFRLWKDEPTMDRTCPFLDKIYQEDIFPCLTFSKSELASAVLEAVENNTLSIEPVGLQPIRFVKASAVECGGPKKCALTGQSKSCKHRIKLGDSSNYYYISPFCRYRITSVCNFFTYIRYIQQGLVKQQDVDQMFWEVMQLRKEMSLAKLGYFKEEL
Clonality
Polyclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for RAB3IP Antibody - Azide and BSA Free
Western Blot: RAB3IP Antibody [H00117177-B01P]
Western Blot: RAB3IP Antibody [H00117177-B01P] - Analysis of RAB3IP expression in rat brain.
Chromatin Immunoprecipitation: RAB3IP Antibody [H00117177-B01P] - 5dpf neuromast immunostained for Rab3IP (red) and counterstained with phalloidin (green). Image courtesy of customer.
Western Blot: RAB3IP Antibody [H00117177-B01P]
Western Blot: RAB3IP Antibody [H00117177-B01P] - Analysis of RAB3IP expression in human placenta.Western Blot: RAB3IP Antibody [H00117177-B01P]
Western Blot: RAB3IP Antibody [H00117177-B01P] - Analysis of RAB3IP expression in transfected 293T cell line by RAB3IP polyclonal antibody. Lane 1: RAB3IP transfected lysate(50.6 KDa). Lane 2: Non-transfected lysate.Applications for RAB3IP Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for Western Blot, Functional. Use in ICC/IF was reported in scientific literature (PMID: 28883094).
Reviewed Applications
Read 1 review rated 4 using H00117177-B01P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RAB3IP
Alternate Names
FLJ14660, FLJ22548, MGC71495, RAB3A interacting protein (rabin3), rab-3A-interacting protein, Rab3A-interacting protein, RABIN3, Rabin-3, SSX2 interacting protein, SSX2-interacting protein
Gene Symbol
RAB3IP
UniProt
Additional RAB3IP Products
Product Documents for RAB3IP Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RAB3IP Antibody - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for RAB3IP Antibody - Azide and BSA Free
Customer Reviews for RAB3IP Antibody - Azide and BSA Free (1)
4 out of 5
1 Customer Rating
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Application: ImmunofluorescenceSample Tested: whole mount zebrafishSpecies: ZebrafishVerified Customer | Posted 02/28/20175dpf neuromast immunostained for Rab3IP (red) and counterstained with phalloidin (green)Whole 3-5dpf fish was fixed at 4⁰C for 4 hours with 4% PFA, 4% sucrose and 0.01% Tween 20 in PBS and then rinsed twice with PBSDT. Primary antibody was diluted in PBSDT containing 3% BSA and 6% FBS at 1:200 dilution and samples incubated overnight with rocking at 4⁰C. After several washes larvae were incubated with AlexaFluor 594-mouse conjugated antibody and AlexaFluor 488-phalloidin
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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