RAC3 Antibody (JE56-48)

Novus Biologicals | Catalog # NBP3-32888

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE56-48 expressed in HEK293
View all RAC3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human RAC3 aa 71-170 / 192. (Uniprot: P60763)

Localization

Cytoplasm, Endomembrane system, Cell projection, lamellipodium, Cytoplasm, perinuclear region, Cell membrane, cytoskeleton.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAC3 Antibody (JE56-48)

RAC3 Antibody (JE56-48)

Western Blot: RAC3 Antibody (JE56-48) [NBP3-32888]

Western Blot: RAC3 Antibody (JE56-48) [NBP3-32888] - Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (NBP3-32888) at 1/2,000 dilution.

Lane 1: HEK-293 cell lysate (20 ug/Lane)
Lane 2: JAR cell lysate (20 ug/Lane)
Lane 3: HUVEC cell lysate (20 ug/Lane)
Lane 4: PC-12 cell lysate (20 ug/Lane)
Lane 5: SH-SY5Y cell lysate (15 ug/Lane)
Lane 6: C2C12 cell lysate (15 ug/Lane)

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32888) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for RAC3 Antibody (JE56-48)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAC3

The protein encoded by the RAC3 gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. (provided by RefSeq)

Long Name

Ras-related C3 botulinum toxin substrate 3

Alternate Names

p21-Rac3

Gene Symbol

RAC3

Additional RAC3 Products

Product Documents for RAC3 Antibody (JE56-48)

Certificate of Analysis

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Product Specific Notices for RAC3 Antibody (JE56-48)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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