RAC3 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00005881-D01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

RAC3 (NP_005043.1, 1 a.a. - 192 a.a.) full-length human protein. MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPHTPILLVGTKLDLRDDKDTIERLRDKKLAPITYPQGLAMAREIGSVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKPGKKCTVF

Specificity

Reacts with ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for RAC3 Antibody - Azide and BSA Free

Western Blot: RAC3 Antibody [H00005881-D01P]

Western Blot: RAC3 Antibody [H00005881-D01P]

Western Blot: RAC3 Antibody [H00005881-D01P] - Analysis of RAC3 expression in transfected 293T cell line by RAC3 polyclonal antibody.Lane 1: RAC3 transfected lysate(21.40 KDa).Lane 2: Non-transfected lysate.

Applications for RAC3 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against transfected lysate in WB and as a detection antibody in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAC3

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. [provided by RefSeq]

Long Name

Ras-related C3 botulinum toxin substrate 3

Alternate Names

p21-Rac3

Entrez Gene IDs

5881 (Human)

Gene Symbol

RAC3

Additional RAC3 Products

Product Documents for RAC3 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RAC3 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for RAC3 Antibody - Azide and BSA Free

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  • Q: I have a customer who is interested this RAC3 antibody (Cat#H00005881-D01P) for Western blotting. They were asking how specific this antibody is for RAC3 (versus other RAC proteins - with which RAC3 shares a high level of sequence similarity)? Does this antibody cross-react with RAC 1 and 2? Are there any western blot images of non-transfected, whole cell lysates? (the example image on website has transfected cell line expression on Western blot).

    A: This antibody is produced by a Taiwanese company called Abnova and we distribute for them. We do no in-house testing or development on their products. The only images available are the ones that Abnova has generated. This is a polyclonal antibody and the immunogen is full-length RAC3. RAC3 shares 94% and 89% similarity with RAC1 and RAC2, respectively. Chances of cross-reactivity with other RAC proteins is quite high. This is true for probably any polyclonal antibody commercially produced against RAC3. A monoclonal is probably the best bet for this target.

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