Rad21 Antibody (BLR052F)

Novus Biologicals | Catalog # NBP3-14677

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR052F
View all Rad21 Primary Antibodies »

Product Specifications

Immunogen

between 581 and 631 (C-term)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Rad21 Antibody (BLR052F)

Western Blot: Rad21 Antibody (BLR052F) [NBP3-14677]

Western Blot: Rad21 Antibody (BLR052F) [NBP3-14677]

Western Blot: Rad21 Antibody [NBP3-14677] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiRad21 recombinant monoclonal antibody used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 3 seconds.
Western Blot: Rad21 Antibody (BLR052F) [NBP3-14677]

Western Blot: Rad21 Antibody (BLR052F) [NBP3-14677]

Western Blot: Rad21 Antibody [NBP3-14677] - Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, RKO,GaMG, MCF-7, A-549, and U2OS cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-Rad21recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 seconds. Lower panel: Rabbit antiMED1 recombinant monoclonal antibody.

Applications for Rad21 Antibody (BLR052F)

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Rad21

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Alternate Names

double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596

Gene Symbol

RAD21

Additional Rad21 Products

Product Documents for Rad21 Antibody (BLR052F)

Certificate of Analysis

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Download SDS

Product Specific Notices for Rad21 Antibody (BLR052F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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