Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rad21 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad21 Antibody - BSA Free

Rad21 Antibody [NB100-203] - Detection of human and mouse Rad21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Rad21 antibody NB100-203 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rad21 Antibody [NB100-203] - Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Rad21 antibody NB100-203 (Lot NB100-203-8) used for IP at 6 ug per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (lot NB100-203-6). For blotting immunoprecipitated Rad21, rabbit anti-Rad21 recombinant monoclonal antibody NB100-203 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.Previous

Applications for Rad21 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2- 10 ug/mg of lysate

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rad21

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Alternate Names

double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596

Gene Symbol

RAD21

Additional Rad21 Products

Product Documents for Rad21 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rad21 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rad21 Antibody - BSA Free

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