Rap1A/B Antibody (JE35-61)

Novus Biologicals | Catalog # NBP3-32893

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE35-61 expressed in HEK293
View all Rap1A/B Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Rap1A/B aa 93-142 / 184. (Uniprot: P62834)

Localization

Cell membrane, Cytoplasm, Cytoplasm, perinuclear region, Cell junction, Early endosome; Cell membrane, Cytoplasm, cytosol, Cell junction.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rap1A/B Antibody (JE35-61)

Rap1A/B Antibody (JE35-61)

Western Blot: Rap1A/B Antibody (JE35-61) [NBP3-32893]

Western Blot: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Western blot analysis of Rap1A/B on different lysates with Rabbit anti-Rap1A/B antibody (NBP3-32893) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: HEK-293 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 20 kDa
Observed band size: 20 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32893) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Rap1A/B Antibody (JE35-61)

Flow Cytometry: Rap1A/B Antibody (JE35-61) [NBP3-32893]

Flow Cytometry: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Flow cytometric analysis of HEK-293 cells labeling Rap1A/B.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32893, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications for Rap1A/B Antibody (JE35-61)

Application
Recommended Usage

Flow Cytometry

1:500-1:1000

Western Blot

1:1000-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rap1A/B

Ras-related Protein 1A (Rap1A) and Ras-related Protein 1B (Rap1B) are membrane-localized members of the Ras small GTPase superfamily of proteins. Rap1A and Rap1B are 184 amino acid (aa) propeptides that are processed into mature 181 aa proteins each with a predicted molecular mass of 21 kDa. Rap1A appears to play a vital role in the cardiovascular system where it regulates cell attachment, migration, and cell-cell junction formation. Rap1A counteracts the mitogenic functions of Ras and several reports suggest that it mediates the progression of multiple cancers where it may regulate metastasis. Rap1B has also been shown to participate in the organization of the vascular lumen by regulating endothelial cell polarity. It also plays a significant role in platelet function, where it mediates adhesion signaling.

Long Name

Ras-related Protein 1A/B

Alternate Names

RAP1A, RAP1B

Gene Symbol

RAP1A

Additional Rap1A/B Products

Product Documents for Rap1A/B Antibody (JE35-61)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rap1A/B Antibody (JE35-61)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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