Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 76805
View all Agrin Primary Antibodies »

Product Specifications

Specificity

Detects rat Agrin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Rat Agrin Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Agrin

Agrin is a 400-600 kDa heparan sulfate proteoglycan component of the extracellular matrix. The N-terminal half of rat Agrin, which mediates ECM interactions, contains nine Kazal-type protease inhibitor domains, two Laminin EGF-like domains, and one SEA domain. The C-terminal half contains four EGF-like repeats and three Laminin globular G domains. Human Agrin also contains a Laminin-binding N-terminal Agrin domain (NtA), and mouse and chick Agrin include the NtA domain only by the use of an alternate promoter. Additional isoforms are generated by alternate splicing at sites Y and Z in the C-terminal half of rat Agrin (known as A and B, respectively in chick). Agrin isoforms that contain an insert at site Z (Z+ forms) are known as neural Agrin and are selectively produced by motoneurons. Other isoforms are known as muscle Agrin and are additionally expressed in non-neuronal tissues, particularly in basement membranes of the lung and kidney (1‑3). This recombinant protein consists of the C-terminal half of rat Agrin and contains a nine amino acid (aa) insert at the Z site. It shares 59%, 80%, and 94% aa sequence identity with comparable regions of chick, human, and mouse Agrin, respectively. The C-terminal half of Z- and Z+ Agrin binds to alpha -Dystroglycan and mediates adhesion between motoneurons and myotubes at the neuromuscular junction (NMJ) (4‑6). In contrast, only Z+ Agrin is effective at inducing clustering of the postsynaptic Acetylcholine Receptor (AChR) and presynaptic motoneuron differentiation (7, 8). Agrin-induced AChR clustering requires a myotube receptor complex that contains alpha -Dystroglycan, MuSK, and LRP4 (4, 9‑11). Agrin exhibits many functions in addition to NMJ development. It is enriched in senile Alzheimer’s disease plaques where it binds the A beta (1-40) peptide and promotes amyloid fibril formation (12). It regulates neuronal excitability by binding and inhibiting the alpha 3 subunit of the neuronal Na/K ATPase (13). It functions as an epithelial cell attachment receptor for HIV-1 through interactions with the gp41 coat protein (14). During T cell activation, Agrin contributes to formation of the immunological synapse and regulates the threshold of T cell activation (15).

References

  1. Jury, E.C. and P.S. Kabouridis (2010) Arthritis Res. Ther. 12:205.
  2. Bezakova, G. and M.A. Ruegg (2003) Nat. Rev. Mol. Cell Biol. 4:295.
  3. Rupp, F. et al. (1991) Neuron 6:811.
  4. Gee, S.H. et al. (1994) Cell 77:675.
  5. Sugiyama, J. et al. (1994) Neuron 13:103.
  6. Gesemann, M. et al. (1998) J. Biol. Chem. 273:600.
  7. Burgess, R.W. et al. (1999) Neuron 23:33.
  8. Ferns, M.J. et al. (1993) Neuron 11:491.
  9. Glass, D.J. et al. (1996) Cell 85:513.
  10. Kim, N. et al. (2008) Cell 135:334.
  11. Zhang, B. et al. (2008) Neuron 60:285.
  12. Cotman, S.L. et al. (2000) Mol. Cell. Neurosci. 15:183.
  13. Hilgenberg, L.G.W. et al. (2006) Cell 125:359.
  14. Alfsen, A. et al. (2005) Mol. Biol. Cell 16:4267.
  15. Khan, A.A. et al. (2001) Science 292:1681.

Alternate Names

AGRN

Entrez Gene IDs

375790 (Human); 11603 (Mouse); 25592 (Rat)

Gene Symbol

AGRN

UniProt

P25304

Additional Agrin Products

Product Documents for Rat Agrin Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat Agrin Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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