Recombinant Feline IL-12 Protein

Formulations:
Catalog # Availability Size / Price Qty
1954-FL-025
Product Details
Citations (2)
FAQs
Reviews

Recombinant Feline IL-12 Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 1-5 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived feline IL-12 protein
Feline IL-12 p40
(Ile23 - Ser329) (Glu167Gly)
Accession # O02744
10-His tag
Feline IL-12 p35
(Arg26 - Ser222) (Ala221Ser)
Accession # O02743
10-His tag
N-terminus C-terminus
N-terminal Sequence
Analysis
Arg26 (p35) & Ile23 (p40)
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
23.7 kDa (p35), 36.2 kDa (p40)
SDS-PAGE
31-33 kDa and 41-45 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1954-FL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1954-FL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12

Interleukin 12 (IL-12) is a 75 kDa heterodimeric glycoprotein that is composed of the disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits. Whereas p35 is an alpha -helical protein related to IL-6, the p40 subunit belongs to the type I cytokine receptor family. The feline IL-12 p35 gene encodes a 222 amino acids (aa) precursor protein with a 25 aa signal peptide. Mature feline p35 contains four potential N-linked glycosylation sites and one intrachain disulfide bond. p35 is synthesized constitutively but can be upregulated by the class II MHC-TCR interaction. p35 is never secreted unbound to p40. Mature feline p35 shows 91%, 88%, 86%, 57%, 56% and 75% aa identity with canine, human, porcine, rat, mouse and guinea pig p35, respectively. The feline p40 gene encodes a secreted 329 aa precursor protein with a 22 aa signal peptide. Mature feline p40 contains one fibronectin type III and one Ig C2-like domain. It is known to exist as a monomer, a homodimer, and as heterodimers linked either to p35 to form IL-12, or to p19 to create IL-23. p40 synthesis is upregulated by many different inflammatory signals including LPS and CpG. Mature feline p40 shows 94%, 85%, 85%, 67%, 67% and 76% aa identity to canine, human, porcine, rat, mouse and guinea pig p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The signaling receptor for IL-12 is composed of two type I transmembrane glycoproteins belonging to the type I cytokine receptor superfamily. IL-12 receptor beta 1 (IL-12 R beta 1) is 100 kDa protein that serves as the principal binding site for the p40 subunit. IL-12 receptor beta 2 (IL-12 R beta 2) is 130 kDa protein that interacts primarily with p35 and functions as the signal transducing component. IL-12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, promotes IgG production (in mice), and potentiates the expansion and late activation of Th1 CD4+ T cells (1 - 5).

References
  1. Schijns, V.E. et al. (1997) Immunogenetics 45:462.
  2. Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
  3. Trinchieri, G. et al. (2003) Immunity 19:641.
  4. Brombacher, F. et al. (2003) Trends Immunol. 24:207.
  5. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Long Name
Interleukin 12
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)
Alternate Names
CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

Citations for Recombinant Feline IL-12 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Immunogenicity and Efficacy of a Novel Multi-Antigenic Peptide Vaccine Based on Cross-Reactivity between Feline and Human Immunodeficiency Viruses
    Authors: B Sahay, AM Aranyos, M Mishra, AC McAvoy, MM Martin, R Pu, S Shiomitsu, K Shiomitsu, MJ Dark, MP Sanou, SR Roff, MH Rathore, JK Yamamoto
    Viruses, 2019;11(2):.
    Species: Feline
    Sample Types:
    Applications: In Vivo
  2. Feline Infectious Peritonitis as a Systemic Inflammatory Disease: Contribution of Liver and Heart to the Pathogenesis
    Authors: AJ Malbon, S Fonfara, ML Meli, S Hahn, H Egberink, A Kipar
    Viruses, 2019;11(12):.
    Species: Feline
    Sample Types: Protein
    Applications: ELISA Standard

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