Recombinant Human BMP-9 Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
3209-BP-010/CF
Recombinant Human BMP-9 Protein, CF Data
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Product Details
Citations (35)
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Recombinant Human BMP-9 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.4-1.6 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-9 protein
Ser320-Arg429
Accession #
N-terminal Sequence
Analysis
Ser320
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.1 kDa (monomer)
SDS-PAGE
13 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3209-BP/CF

Formulation Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution

It is recommended that sterile 4 mM HCl be added to the vial to prepare a working stock solution of no less than 100 μg/mL. The carrier-free protein should be used immediately upon dilution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

3209-BP

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human BMP-9 (Catalog # 3209-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50for this effect is 0.4-1.6 ng/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human BMP-9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 23 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4 - 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8 - 10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
  4. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
  5. Song, J.J. et al. (1995) Endocrinology 136:4293.
  6. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
  7. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
  8. Lopez-Coviella, I. et al. (2000) Science 289:313.
  9. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984.
  10. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
  11. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
  12. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
  13. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
Long Name
Bone Morphogenetic Protein 9
Entrez Gene IDs
2658 (Human); 12165 (Mouse)
Alternate Names
BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2

Citations for Recombinant Human BMP-9 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. CD105 overexpression mediates drug-resistance in choriocarcinoma cells through BMP9/Smad pathway
    Authors: X Wang, L Zong, W Wang, J Yang, Y Xiang
    J Cancer, 2020;11(2):272-283.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. BMP9 stimulates joint regeneration at digit amputation wounds in mice
    Authors: L Yu, LA Dawson, M Yan, K Zimmel, YL Lin, CP Dolan, M Han, K Muneoka
    Nat Commun, 2019;10(1):424.
    Species: Mouse
    Sample Types: Beads
    Applications: In Vivo
  3. The transcriptomic and epigenetic map of vascular quiescence in the continuous lung endothelium
    Authors: K Schlereth, D Weichenhan, T Bauer, T Heumann, E Giannakour, D Lipka, S Jaeger, M Schlesner, P Aloy, R Eils, C Plass, HG Augustin
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Endoglin interacts with VEGFR2 to promote angiogenesis
    Authors: H Tian, JJ Huang, C Golzio, X Gao, M Hector-Gre, N Katsanis, GC Blobe
    FASEB J., 2018;0(0):fj201700867RR.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Bone Morphogenetic Protein 9 Regulates Early Lymphatic-Specified Endothelial Cell Expansion during Mouse Embryonic Stem Cell Differentiation
    Authors: M Subileau, G Merdzhanov, D Ciais, V Collin-Fau, JJ Feige, S Bailly, D Vittet
    Stem Cell Reports, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A heterodimer formed by bone morphogenetic protein 9 (BMP9) and BMP10 provides most BMP biological activity in plasma
    Authors: E Tillet, M Ouarné, A Desroches-, C Mallet, M Subileau, R Didier, A Lioutsko, G Belthier, JJ Feige, S Bailly
    J. Biol. Chem., 2018;0(0):.
    Applications: Western Blot
  7. Hepcidin is regulated by promoter-associated histone acetylation and HDAC3
    Authors: SR Pasricha, PJ Lim, TL Duarte, C Casu, D Oosterhuis, K Mleczko-Sa, M Suciu, AR Da Silva, K Al-Hourani, J Arezes, K McHugh, S Gooding, JN Frost, K Wray, A Santos, G Porto, E Repapi, N Gray, SJ Draper, N Ashley, E Soilleux, P Olinga, MU Muckenthal, JR Hughes, S Rivella, TA Milne, AE Armitage, H Drakesmith
    Nat Commun, 2017;8(1):403.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. Dual targeting of vascular endothelial growth factor and bone morphogenetic protein-9/10 impairs tumor growth through inhibition of angiogenesis
    Authors: Y Akatsu, Y Yoshimatsu, T Tomizawa, K Takahashi, A Katsura, K Miyazono, T Watabe
    Cancer Sci, 2017;108(1):151-155.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology
    Authors: K Sakamoto, Y Kanematsu-, Y Kamada, M Oka, T Ohnishi, M Miwa, T Asami, H Inooka
    Biochem Biophys Rep, 2017;11(0):33-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Mutual interaction between endothelial cells and mural cells enhances BMP9 signaling in endothelial cells
    Authors: Y Tachida, N Izumi, T Sakurai, H Kobayashi
    Biol Open, 2017;6(3):370-380.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Bone morphogenetic protein-9 increases the functional expression of organic anion transporting polypeptide 1a4 at the blood-brain barrier via the activin receptor-like kinase-1 receptor
    Authors: W Abdullahi, H Brzica, K Ibbotson, TP Davis, PT Ronaldson
    J. Cereb. Blood Flow Metab., 2017;0(0):271678X177029.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  12. Peptides derived from the knuckle epitope of BMP-9 induce the cholinergic differentiation and inactivate GSk3beta in human SH-SY5Y neuroblastoma cells
    Authors: MA Lauzon, O Drevelle, N Faucheux
    Sci Rep, 2017;7(1):4695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Bone morphogenetic protein (BMP) 9 and BMP10 enhance tumor necrosis factor-?-induced monocyte recruitment to the vascular endothelium mainly via activin receptor-like kinase 2
    Authors: CG Mitrofan, SL Appleby, GB Nash, Z Mallat, ER Chilvers, PD Upton, NW Morrell
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. BMP-9 interferes with liver regeneration and promotes liver fibrosis
    Authors: K Breitkopf-, C Meyer, C König, H Gaitantzi, A Addante, M Thomas, E Wiercinska, C Cai, Q Li, F Wan, C Hellerbran, NA Valous, M Hahnel, C Ehlting, JG Bode, S Müller-Boh, U Klingmülle, J Altenöder, I Ilkavets, MJ Goumans, LJ Hawinkels, SJ Lee, M Wieland, C Mogler, MP Ebert, B Herrera, H Augustin, A Sánchez, S Dooley, P Ten Dijke
    Gut, 2017;0(0):.
    Applications: ELISA (Standard)
  15. MxA Is a Novel Regulator of Endosome-Associated Transcriptional Signaling by Bone Morphogenetic Proteins 4 and 9 (BMP4 and BMP9)
    PLoS ONE, 2016;11(11):e0166382.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells.
    Authors: Kraehling J, Chidlow J, Rajagopal C, Sugiyama M, Fowler J, Lee M, Zhang X, Ramirez C, Park E, Tao B, Chen K, Kuruvilla L, Larrivee B, Folta-Stogniew E, Ola R, Rotllan N, Zhou W, Nagle M, Herz J, Williams K, Eichmann A, Lee W, Fernandez-Hernando C, Sessa W
    Nat Commun, 2016;7(0):13516.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  17. Hyaluronic Acid Gel-Based Scaffolds as Potential Carrier for Growth Factors: An In Vitro Bioassay on Its Osteogenic Potential
    J Clin Med, 2016;5(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Bone Morphogenetic Protein 9 Enhances Lipopolysaccharide-Induced Leukocyte Recruitment to the Vascular Endothelium
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. BMP9 Crosstalk with the Hippo Pathway Regulates Endothelial Cell Matricellular and Chemokine Responses.
    Authors: Young K, Tweedie E, Conley B, Ames J, FitzSimons M, Brooks P, Liaw L, Vary C
    PLoS ONE, 2015;10(4):e0122892.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Serum induces transcription of Hey1 and Hey2 genes by Alk1 but not Notch signaling in endothelial cells.
    Authors: Woltje K, Jabs M, Fischer A
    PLoS ONE, 2015;10(3):e0120547.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus.
    Authors: Levet S, Ouarne M, Ciais D, Coutton C, Subileau M, Mallet C, Ricard N, Bidart M, Debillon T, Faravelli F, Rooryck C, Feige J, Tillet E, Bailly S
    Proc Natl Acad Sci U S A, 2015;112(25):E3207-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology.
    Authors: Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, Ricci-Vitiani L, D'Alessandris Q, Morgante L, Giannetti S, Larocca L, Todaro M, Benfante A, Colorito M, Stassi G, De Maria R, Rowlinson S, Stancato L
    PLoS ONE, 2015;10(4):e0125697.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Bone morphogenetic protein-9 suppresses growth of myeloma cells by signaling through ALK2 but is inhibited by endoglin.
    Authors: Olsen O, Wader K, Misund K, Vatsveen T, Ro T, Mylin A, Turesson I, Stordal B, Moen S, Standal T, Waage A, Sundan A, Holien T
    Blood Cancer J, 2014;4(0):e196.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Src-mediated post-translational regulation of endoglin stability and function is critical for angiogenesis.
    Authors: Pan C, Kumar S, Shah N, Hoyt D, Hawinkels L, Mythreye K, Lee N
    J Biol Chem, 2014;289(37):25486-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits.
    Authors: Liu Y, Weick J, Liu H, Krencik R, Zhang X, Ma L, Zhou G, Ayala M, Zhang S
    Nat Biotechnol, 2013;31(5):440-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
    Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI
    Blood, 2012;119(21):5037-47.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  27. BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
    Blood, 2012;119(25):6162-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. BMP9 regulates endoglin-dependent chemokine responses in endothelial cells.
    Authors: Young, Kira, Conley, Barbara, Romero, Diana, Tweedie, Eric, O'Neill, Christin, Pinz, Ilka, Brogan, Louise, Lindner, Volkhard, Liaw, Lucy, Vary, Calvin P
    Blood, 2012;120(20):4263-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs).
    Authors: Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L
    PLoS ONE, 2012;7(9):e46307.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  31. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  32. Functional analysis of the BMP9 response of ALK1 mutants from HHT2 patients: a diagnostic tool for novel ACVRL1 mutations.
    Authors: Ricard N, Bidart M, Mallet C
    Blood, 2010;116(9):1604-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
    Authors: Herrera B, Inman GJ,
    BMC Cell Biol., 2009;10(0):20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells.
    Authors: David L, Mallet C, Mazerbourg S, Feige JJ, Bailly S
    Blood, 2007;109(5):1953-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Iron transferrin regulates hepcidin synthesis in primary hepatocyte culture through hemojuvelin and BMP2/4.
    Authors: Lin L, Valore EV, Nemeth E, Goodnough JB, Gabayan V, Ganz T
    Blood, 2007;110(6):2182-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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