Recombinant Human BMP-9 Protein, CF

  (30 citations)     
Datasheet / CoA / SDS
Product Details
Citations (30)
FAQs
Reviews
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.4-1.6 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived human BMP-9 protein
    Ser320-Arg429
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser320
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    12.1 kDa (monomer)
  • SDS-PAGE
    13 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3209-BP/CF
 
3209-BP
Formulation Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
 
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution

It is recommended that sterile 4 mM HCl be added to the vial to prepare a working stock solution of no less than 100 μg/mL. The carrier-free protein should be used immediately upon dilution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.

 
Reconstitution Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human BMP-9 (Catalog # 3209-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.4-1.6 ng/mL.
1 μg/lane of Recombinant Human BMP-9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 23 kDa, respectively.
Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4 - 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8 - 10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

  • References:
    1. Chen, D. et al. (2004) Growth Factors 22:233.
    2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
    3. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
    4. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
    5. Song, J.J. et al. (1995) Endocrinology 136:4293.
    6. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
    7. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
    8. Lopez-Coviella, I. et al. (2000) Science 289:313.
    9. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984.
    10. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
    11. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
    12. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
    13. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
  • Long Name:
    Bone Morphogenetic Protein 9
  • Entrez Gene IDs:
    2658 (Human); 12165 (Mouse)
  • Alternate Names:
    BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Bone morphogenetic protein-9 increases the functional expression of organic anion transporting polypeptide 1a4 at the blood-brain barrier via the activin receptor-like kinase-1 receptor
    Authors: W Abdullahi, H Brzica, K Ibbotson, TP Davis, PT Ronaldson
    J. Cereb. Blood Flow Metab., 2017;0(0):271678X177029.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  2. Mutual interaction between endothelial cells and mural cells enhances BMP9 signaling in endothelial cells
    Authors: Y Tachida, N Izumi, T Sakurai, H Kobayashi
    Biol Open, 2017;6(3):370-380.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. BMP-9 interferes with liver regeneration and promotes liver fibrosis
    Authors: K Breitkopf-, C Meyer, C König, H Gaitantzi, A Addante, M Thomas, E Wiercinska, C Cai, Q Li, F Wan, C Hellerbran, NA Valous, M Hahnel, C Ehlting, JG Bode, S Müller-Boh, U Klingmülle, J Altenöder, I Ilkavets, MJ Goumans, LJ Hawinkels, SJ Lee, M Wieland, C Mogler, MP Ebert, B Herrera, H Augustin, A Sánchez, S Dooley, P Ten Dijke
    Gut, 2017;0(0):.
    Application: ELISA Standard
  4. Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology
    Authors: K Sakamoto, Y Kanematsu-, Y Kamada, M Oka, T Ohnishi, M Miwa, T Asami, H Inooka
    Biochem Biophys Rep, 2017;11(0):33-39.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Hepcidin is regulated by promoter-associated histone acetylation and HDAC3
    Authors: SR Pasricha, PJ Lim, TL Duarte, C Casu, D Oosterhuis, K Mleczko-Sa, M Suciu, AR Da Silva, K Al-Hourani, J Arezes, K McHugh, S Gooding, JN Frost, K Wray, A Santos, G Porto, E Repapi, N Gray, SJ Draper, N Ashley, E Soilleux, P Olinga, MU Muckenthal, JR Hughes, S Rivella, TA Milne, AE Armitage, H Drakesmith
    Nat Commun, 2017;8(1):403.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  6. Peptides derived from the knuckle epitope of BMP-9 induce the cholinergic differentiation and inactivate GSk3beta in human SH-SY5Y neuroblastoma cells
    Authors: MA Lauzon, O Drevelle, N Faucheux
    Sci Rep, 2017;7(1):4695.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Bone morphogenetic protein (BMP) 9 and BMP10 enhance tumor necrosis factor-?-induced monocyte recruitment to the vascular endothelium mainly via activin receptor-like kinase 2
    Authors: CG Mitrofan, SL Appleby, GB Nash, Z Mallat, ER Chilvers, PD Upton, NW Morrell
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Dual targeting of vascular endothelial growth factor and bone morphogenetic protein-9/10 impairs tumor growth through inhibition of angiogenesis
    Authors: Y Akatsu, Y Yoshimatsu, T Tomizawa, K Takahashi, A Katsura, K Miyazono, T Watabe
    Cancer Sci, 2017;108(1):151-155.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells.
    Authors: Kraehling J, Chidlow J, Rajagopal C, Sugiyama M, Fowler J, Lee M, Zhang X, Ramirez C, Park E, Tao B, Chen K, Kuruvilla L, Larrivee B, Folta-Stogniew E, Ola R, Rotllan N, Zhou W, Nagle M, Herz J, Williams K, Eichmann A, Lee W, Fernandez-Hernando C, Sessa W
    Nat Commun, 2016;7(0):13516.
    Species: Human
    Sample Type: Recombinant Protein
    Application: EnzAct
  10. Hyaluronic Acid Gel-Based Scaffolds as Potential Carrier for Growth Factors: An In Vitro Bioassay on Its Osteogenic Potential
    J Clin Med, 2016;5(12):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. MxA Is a Novel Regulator of Endosome-Associated Transcriptional Signaling by Bone Morphogenetic Proteins 4 and 9 (BMP4 and BMP9)
    PLoS ONE, 2016;11(11):e0166382.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Bone Morphogenetic Protein 9 Enhances Lipopolysaccharide-Induced Leukocyte Recruitment to the Vascular Endothelium
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells
    Nat Commun, 2016;7(0):13516.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus.
    Authors: Levet S, Ouarne M, Ciais D, Coutton C, Subileau M, Mallet C, Ricard N, Bidart M, Debillon T, Faravelli F, Rooryck C, Feige J, Tillet E, Bailly S
    Proc Natl Acad Sci U S A, 2015;112(25):E3207-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. BMP9 Crosstalk with the Hippo Pathway Regulates Endothelial Cell Matricellular and Chemokine Responses.
    Authors: Young K, Tweedie E, Conley B, Ames J, FitzSimons M, Brooks P, Liaw L, Vary C
    PLoS ONE, 2015;10(4):e0122892.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology.
    Authors: Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, Ricci-Vitiani L, D'Alessandris Q, Morgante L, Giannetti S, Larocca L, Todaro M, Benfante A, Colorito M, Stassi G, De Maria R, Rowlinson S, Stancato L
    PLoS ONE, 2015;10(4):e0125697.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Serum induces transcription of Hey1 and Hey2 genes by Alk1 but not Notch signaling in endothelial cells.
    Authors: Woltje K, Jabs M, Fischer A
    PLoS ONE, 2015;10(3):e0120547.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Bone morphogenetic protein-9 suppresses growth of myeloma cells by signaling through ALK2 but is inhibited by endoglin.
    Authors: Olsen O, Wader K, Misund K, Vatsveen T, Ro T, Mylin A, Turesson I, Stordal B, Moen S, Standal T, Waage A, Sundan A, Holien T
    Blood Cancer J, 2014;4(0):e196.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Src-mediated post-translational regulation of endoglin stability and function is critical for angiogenesis.
    Authors: Pan C, Kumar S, Shah N, Hoyt D, Hawinkels L, Mythreye K, Lee N
    J Biol Chem, 2014;289(37):25486-96.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits.
    Authors: Liu Y, Weick J, Liu H, Krencik R, Zhang X, Ma L, Zhou G, Ayala M, Zhang S
    Nat Biotechnol, 2013;31(5):440-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
    Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI
    Blood, 2012;119(21):5037-47.
    Species: Human
    Sample Type: Cell Lysates
    Application: Bioassay
  22. Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs).
    Authors: Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L
    PLoS ONE, 2012;7(9):e46307.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Surface Plasmon Resonance
  23. BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
    Blood, 2012;119(25):6162-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. BMP9 regulates endoglin-dependent chemokine responses in endothelial cells.
    Authors: Young, Kira, Conley, Barbara, Romero, Diana, Tweedie, Eric, O'Neill, Christin, Pinz, Ilka, Brogan, Louise, Lindner, Volkhard, Liaw, Lucy, Vary, Calvin P
    Blood, 2012;120(20):4263-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Surface Plasmon Resonance
  27. Functional analysis of the BMP9 response of ALK1 mutants from HHT2 patients: a diagnostic tool for novel ACVRL1 mutations.
    Authors: Ricard N, Bidart M, Mallet C
    Blood, 2010;116(9):1604-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
    Authors: Herrera B, Inman GJ,
    BMC Cell Biol., 2009;10(0):20.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  29. Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells.
    Authors: David L, Mallet C, Mazerbourg S, Feige JJ, Bailly S
    Blood, 2007;109(5):1953-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  30. Iron transferrin regulates hepcidin synthesis in primary hepatocyte culture through hemojuvelin and BMP2/4.
    Authors: Lin L, Valore EV, Nemeth E, Goodnough JB, Gabayan V, Ganz T
    Blood, 2007;110(6):2182-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Have you used Recombinant Human BMP-9 Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

  • Formulations
Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee