Recombinant Human BTN2A1 Fc Chimera Protein, CF

R&D Systems | Catalog # 11521-BT

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human BTN2A1 Fc Chimera Protein (11521-BT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q7KYR7.3

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BTN2A1 protein
Human BTN2A1
(Gln29-Ala248)
Accession # Q7KYR7.3		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Identity confirmed by protein ID

Predicted Molecular Mass

51 kDa

SDS-PAGE

60-80 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human BTN2A1 Fc Chimera binds to biotinylated DC-SIGN Fc Chimera with a ED50 of 1.00-10.0 μg/mL.

Scientific Data Images for Recombinant Human BTN2A1 Fc Chimera Protein, CF

Recombinant Human BTN2A1 Fc Chimera Protein SEC-MALS.

Recombinant Human BTN2A1 Fc Chimera Protein (Catalog # 11521-BT) has a molecular weight (MW) of 124.5 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Human BTN2A1 Fc Chimera Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human BTN2A1 Fc Chimera Protein(Catalog # 11521-BT) binds to biotinylated DC-SIGN Fc Chimera with a ED50 of 1.00-10.0 μg/mL.

Recombinant Human BTN2A1 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human BTN2A1 Fc Chimera Protein (Catalog # 11521-BT) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa.

Formulation, Preparation, and Storage

11521-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTN2A1

Butyrophilin 2A1 (BTN2A1) is a type I transmembrane glycoprotein of the butyrophilin family within the Ig-superfamily. There are over 13 Butyrophilin and the closely related butyrophilin-like (BTNL) molecules and they have been identified as immune checkpoint receptors involved in modulating T cell function (1,2). Mature human BTN2A1 consists of an extracellular domain (ECD) with two immunoglobulin-like domains (one IgV and one IgC), a transmembrane segment, and a cytoplasmic region with a B30.2 domain (3). Alternative splicing generates additional isoforms of BTN2A1 that lack the first Ig-like domain or the transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (4, 5). BTN2A1 binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (4). A polymorphism of BTN2A1 has been associated with metabolic syndromes, type II diabetes mellitus, chronic kidney disease, and hypertension (6, 7). 

References

  1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
  2. Afrache, H. et al. (2012) Immunogenetics 64:781.
  3. Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
  4. Malcherek, G. et al. (2007) J. Immunol. 179:3804.
  5. Cavaletto, M. et al. (2002) Proteomics 2:850.
  6. Oguri, M. et al.(2011) J. Med. Genet. 48:787.
  7. Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.

Long Name

Butyrophilin Subfamily 2 Member A1

Alternate Names

BT2.1, BTF1

Entrez Gene IDs

11120 (Human)

Gene Symbol

BTN2A1

UniProt

Q7KYR7.3

Additional BTN2A1 Products

Product Documents for Recombinant Human BTN2A1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human BTN2A1 Fc Chimera Protein, CF

For research use only

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