Recombinant Human BTNL8 Fc Chimera Protein, CF

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Catalog # Availability Size / Price Qty
9359-BT-050
Recombinant Human BTNL8 Fc Chimera Protein, CF Data
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Recombinant Human BTNL8 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3+ T cells. The ED50 for this effect is 0.5-2.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human BTNL8 protein
Human BTNL8
(Gln18-Lys238)
Accession # Q6UX41-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln18 inferred from enzymatic pyroglutamate treatment revealing Trp19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52 kDa
SDS-PAGE
53-63 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9359-BT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

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Recombinant Human BTNL8 Fc Chimera (Catalog # 9359-BT) enhances anti-CD3-induced IFN-gamma secretion by mouse CD3+T cells. The ED50for this effect is 0.5-2.5 μg/mL.

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Reconstitution Calculator

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Background: BTNL8

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells. In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of
Ag-specific IgG during the primary but not the secondary immune response (3).

References
  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
  4. Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.
Long Name
Butyrophilin-like 8
Entrez Gene IDs
79908 (Human); 406160 (Rat)
Alternate Names
BTN9.2; BTNL8; butyrophilin-like 8; butyrophilin-like protein 8; UNQ702/PRO1347

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Reviews for Recombinant Human BTNL8 Fc Chimera Protein, CF

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Recombinant Human BTNL8 Fc Chimera Protein, CF
By Anonymous on 03/25/2019
Application: Apoptosis assay

Recombinant Human BTNL8 Fc Chimera Protein, CF
By Anonymous on 10/12/2018
Application: CellProlif

Recombinant Human BTNL8 Fc Chimera Protein, CF
By Anonymous on 07/11/2018
Application: Binding assay/Protein-protein interaction