Recombinant Human BTNL8 Fc Chimera Protein, CF
Recombinant Human BTNL8 Fc Chimera Protein, CF Summary
|Human BTNL8 |
Accession # Q6UX41-1
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human BTNL8 Fc Chimera (Catalog # 9359-BT) enhances anti-CD3-induced IFN-gamma secretion by mouse CD3+T cells. The ED50 for this effect is 0.5-2.5 μg/mL.
8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative
regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I
transmembrane glycoprotein that contains a signal peptide followed by an
extracellular domain (ECD), a transmembrane region and a short cytoplasmic
domain (2). The ECD of human BTNL8 shares 88% sequence identity with the
ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and
BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3).
The complete immunological function of BTNL molecules is only beginning to
emerge. BTNL8 has been shown to be important in initiation of primary immune
responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and
colon cancer (4). BTNL8
are expressed in colon, lung, testis and neutrophils, and its expression is
significantly decreased in ulcerative colitis, colonic tumors as compared to
unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but
not activated T cells. In vitro,
BTNL8 co-stimulates T cell proliferation and cytokine production. In
vivo injections of BTNL8-Fc significantly increases
Ag-specific IgG during the primary but not the secondary immune response (3).
- Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
- Arnett, H.A. et al. (2009) Cytokine 46:370.
- Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
- Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.
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