Recombinant Human BTNL8 Fc Chimera Protein, CF

R&D Systems | Catalog # 9359-BT

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human BTNL8 Fc Chimera Protein (9359-BT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q6UX41-1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all BTNL8 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BTNL8 protein
Human BTNL8
(Gln18-Lys238)
Accession # Q6UX41-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln18 inferred from enzymatic pyroglutamate treatment revealing Trp19

Predicted Molecular Mass

52 kDa

SDS-PAGE

53-63 kDa, reducing conditions

Activity

Measured by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3+ T cells.
The ED50 for this effect is 0.5-2.5 μg/mL.

Reviewed Applications

Read 3 reviews rated 5 using 9359-BT in the following applications:

Scientific Data Images for Recombinant Human BTNL8 Fc Chimera Protein, CF

Recombinant Human BTNL8 Fc Chimera Protein Bioactivity

Recombinant Human BTNL8 Fc Chimera Protein Bioactivity

Recombinant Human BTNL8 Fc Chimera (Catalog # 9359-BT) enhances anti-CD3-induced IFN-gamma secretion by mouse CD3+T cells. The ED50 for this effect is 0.5-2.5 μg/mL.

Formulation, Preparation, and Storage

9359-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTNL8

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells. In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of
Ag-specific IgG during the primary but not the secondary immune response (3).

References

  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
  4. Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.

Long Name

Butyrophilin-like 8

Alternate Names

BTN9.2

Entrez Gene IDs

79908 (Human); 406160 (Rat)

Gene Symbol

BTNL8

UniProt

Q6UX41-1

Additional BTNL8 Products

Product Documents for Recombinant Human BTNL8 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human BTNL8 Fc Chimera Protein, CF

For research use only

Customer Reviews for Recombinant Human BTNL8 Fc Chimera Protein, CF (3)

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  • Recombinant Human BTNL8 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Apoptosis assay
    Verified Customer | Posted 03/25/2019
  • Recombinant Human BTNL8 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Cell Proliferation
    Verified Customer | Posted 10/12/2018
  • Recombinant Human BTNL8 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 07/11/2018

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