Recombinant Human CEACAM-18 Fc Chimera Protein, CF

R&D Systems | Catalog # 9869-CM

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human CEACAM-18 Fc Chimera Protein (9869-CM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

A8MTB9

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CEACAM-18 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CEACAM-18 protein
Human CEACAM-18
(Gln31-His317)
Accession # A8MTB9		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln31 inferred from enzymatic pyroglutamate treatment revealing Ile32.

Predicted Molecular Mass

59 kDa

SDS-PAGE

93-105 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Galectin-3 (Catalog # 8259-GA) is immobilized at 1 μg/mL, 100 μL/well, it binds Recombinant Human CEACAM-18 Fc Chimera. The concentration of Recombinant Human CEACAM-18 Fc Chimera that produces 50% of the optimal binding response is
0.5-5 μg/mL.

Scientific Data Images for Recombinant Human CEACAM-18 Fc Chimera Protein, CF

Recombinant Human CEACAM-18 Fc Chimera Protein Binding Activity

Recombinant Human CEACAM-18 Fc Chimera Protein Binding Activity

When Recombinant Human Galectin-3 (Human Cell-expressed) (Catalog # 8259-GA) is coated at 1 µg/mL, 100 µL/well, Recombinant Human CEACAM-18 Fc Chimera (Catalog # 9869-CM) binds with an ED50 of 0.5-5 µg/mL.

Recombinant Human CEACAM-18 Fc Chimera Protein SDS-PAGE

Recombinant Human CEACAM-18 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CEACAM-18 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 93 -105 kDa and 190- 200 kDa, respectively.

Formulation, Preparation, and Storage

9869-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-18

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18 (CEACAM-18) is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Mature human CEACAM-18 has a 298 amino acid (aa) extracellular domain containing 2 IgC2-like and 1 IgV-like domains, a single transmembrane domain and a short cytoplasmic tail (2). CEACAM-18 is one of only five conserved CEACAMs among mouse, rat, and human (2), but mature human CEACAM-18 has low aa sequence identify with mouse and rat at 60% and 58%, respectively. Originally discovered as a biomarker for colorectal cancer (3), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (4-6). While the exact function of CEACAM-18 has been yet to be elucidated, it may bind pathogen receptors or other immunoregulatory members (6). CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils (7). Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators (8, 9).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser R. et al. (2005) Genomics 86:566.
  3. Gold P and Freedman SO, 1965) J Exp Med 122:467.
  4. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  5. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  6. Kuespert K. et al. (2006) Curr Opin Cell Biol. 18(5):565.
  7. Feuk-Lagerstedt E. et al. (1999) J. Immunol. 163:5592.
  8. Yoon, J. et al. (2007) J. Immunol. 179:8454.
  9. Schröder, A.K. et al. (2006) Hum Immunol. 67:676.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18

Alternate Names

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 18

Entrez Gene IDs

729767 (Human); 72431 (Mouse); 502334 (Rat)

Gene Symbol

CEACAM18

UniProt

A8MTB9

Additional CEACAM-18 Products

Product Documents for Recombinant Human CEACAM-18 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CEACAM-18 Fc Chimera Protein, CF

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

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