Recombinant Human Galectin-3 (Human Cell-expressed), CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. The ED50 for this effect id 0.6-3 μg/mL. Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 3-10 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Ala2-Ile250
  • Accession #
  • N-terminal Sequence
    Analysis

    No results obtained: Ala2 predicted.

  • Predicted Molecular Mass
    25 kDa
  • SDS-PAGE
    26-31 kDa, reducing conditions
8259-GA
 
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human Galectin-3 (Catalog # 8259-GA) supports the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. The ED50 for this effect is 0.6-3 μg/mL, whilecompetitor's protein shows very weak activity.
1 μg/lane of Recombinant Human Galectin-3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 29.9 kDa and 29.8 kDa, respectively.
Background: Galectin-3

Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain (1, 2). Mature human Galectin-3 shares 78% and 79% amino acid (aa) sequence identity with mouse and rat Galectin-3, respectively. Human Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the crosslinking of cell surface oligosacchraides as well as integrin‑dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).

  • References:
    1. Robertson, M.W. et al. (1990) Biochemistry 29:8093.
    2. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
    3. Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181.
    4. Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172.
    5. Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305.
    6. Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616.
    7. Sano, H. et al. (2003) J. Clin. Invest. 112:389.
    8. Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841.
    9. Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264.
    10. Wen, Y. et al. (2006) J. Cell. Biochem. 98:115.
    11. Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177.
    12. Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160.
    13. Kohatsu, L. et al. (2006) J. Immunol. 177:4718.
    14. Nieminen, J. et al. (2008) J. Immunol. 180:2466.
    15. Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288.
    16. Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123.
    17. Sherwi, N. et al. (2012) Future Cardiol. 8:885.
    18. Shin, T. (2013) Acta Histochem. 115:407.
  • Entrez Gene IDs:
    3958 (Human); 16854 (Mouse)
  • Alternate Names:
    AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3
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