CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for human CX3CL1 has been mapped to chromosome 16q.
Recombinant Human CX3CL1/Fractalkine (Full Length) Protein
R&D Systems | Catalog # 365-FR
Key Product Details
- R&D Systems NS0-derived Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (365-FR)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
| Human CX3CL 1 (Gln25 - Arg339) (Ser199Asn) Accession # P78423 |
KQN | 6-His tag |
| N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 20-100 ng/mL.
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CX3CR1.
The ED50 for this effect is 2.5-10 ng/mL.
Reviewed Applications
Read 1 review rated 5 using 365-FR in the following applications:
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 365-FR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 365-FR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: CX3CL1/Fractalkine
References
- Pan, Y. et al. (1997) Nature 387:611.
- Bazan J.F. et al. (1997) Nature 385:640.
- Mackay, C.R. (1997) Current Biology 7:R384.
Alternate Names
Gene Symbol
UniProt
Additional CX3CL1/Fractalkine Products
Product Documents for Recombinant Human CX3CL1/Fractalkine (Full Length) Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human CX3CL1/Fractalkine (Full Length) Protein
For research use only
Related Research Areas
Citations for Recombinant Human CX3CL1/Fractalkine (Full Length) Protein
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Customer Reviews for Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (1)
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Application: Cell migration/motilitySpecies: HumanVerified Customer | Posted 04/24/2025Comparison of NK cell count/µL in the control condition (R10) vs CX3CL1 at 10ng/mL200.000 sorted NK cells were place in the upper chamber of a 24 well plate with transwell inserts (5µm pore size). 650µL of culture media containing 10ng/mL of CX3CL1 were placed in the lower chamber. Cell count was performed by flow cytometry using counting bead after one hour of migration at 37ºC.
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Associated Pathways
Chemokine Superfamily Pathway: Human/Mouse Ligand-Receptor Interactions
