Recombinant Human Dectin-1/CLEC7A Protein, CF

Catalog # Availability Size / Price Qty
1859-DC-050
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human Dectin-1/CLEC7A Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated Laminarin (1, 3-beta -glucan) in a functional ELISA with an estimated KD <5 nM.
Source
Mouse myeloma cell line, NS0-derived human Dectin-1/CLEC7A protein
Thr66-Met201, with an N-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
16.8 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1859-DC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3 - 5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5 - 7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 - 13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15 - 16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29% - 39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

Citations for Recombinant Human Dectin-1/CLEC7A Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Differential susceptibility of Dectin-1 isoforms to functional inactivation by neutrophil and fungal proteases
    Authors: JS Griffiths, A Thompson, M Stott, A Benny, NA Lewis, PR Taylor, J Forton, S Herrick, SJ Orr, EP McGreal
    FASEB J., 2018;0(0):fj201701145R.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins
    Authors: C Leclaire, K Lecointe, PA Gunning, S Tribolo, DW Kavanaugh, A Wittmann, D Latousakis, DA MacKenzie, N Kawasaki, N Juge
    FASEB J., 2018;0(0):fj201700619R.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Force Microscopy
  3. Immunoregulatory Activity of the Natural Product Laminarin Varies Widely as a Result of Its Physical Properties
    Authors: AJ Smith, B Graves, R Child, PJ Rice, Z Ma, DW Lowman, HE Ensley, KT Ryter, JT Evans, DL Williams
    J. Immunol., 2017;0(0):.
    Applications: Bioassay
  4. Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors
    Authors: KP Bene, DW Kavanaugh, C Leclaire, AP Gunning, DA MacKenzie, A Wittmann, ID Young, N Kawasaki, E Rajnavolgy, N Juge
    Front Microbiol, 2017;8(0):321.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. FleA Expression in Aspergillus fumigatus Is Recognized by Fucosylated Structures on Mucins and Macrophages to Prevent Lung Infection
    Authors: SC Kerr, GJ Fischer, M Sinha, O McCabe, JM Palmer, T Choera, F Yun Lim, M Wimmerova, SD Carrington, S Yuan, CA Lowell, S Oscarson, NP Keller, JV Fahy
    PLoS Pathog, 2016;12(4):e1005555.
    Species: Fungus
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
    PLoS ONE, 2015;10(3):e0119244.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Dectin-1/CLEC7A Protein, CF
By Anonymous on 11/24/2017
Application: Binding assay/Protein-protein interaction