Recombinant Human Fc gamma RIIA/CD32a (R167) Protein

Formulations:
Catalog # Availability Size / Price Qty
1330-CD-050
Recombinant Human Fc gamma RIIA/CD32a (R167) Protein Binding Activity
2 Images
Product Details
Citations (9)
FAQs
Reviews

Recombinant Human Fc gamma RIIA/CD32a (R167) Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind human IgG with an estimated Kd <80 nM.
Source
Mouse myeloma cell line, NS0-derived human Fc gamma RIIA/CD32a protein
Ala36-Ile218, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Ala36
Predicted Molecular Mass
22 kDa
SDS-PAGE
32 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1330-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1330-CD/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

Recombinant Human Fc gamma RIIA/CD32a (R167) (Catalog # 1330-CD) binds human IgG with an estimated KD 80 nM.

SDS-PAGE SDS-PAGE View Larger

1 μg/lane of Recombinant Human Fc gamma  RIIA/CD32a (R167) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 32 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: Fc gamma RIIA/CD32a

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1-3). The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).

Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified (1-3). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) (4, 5). Two allelic variants (R167 and H167) of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The H167 allele has been found to have a protective effect against lupus nephritis.

References
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Chi, M. et al. (2002) J. Immunol. 168:1413.
  5. Zuniga, R. et al. (2003) Arthritis Rheum. 48:460.
Long Name
Fc gamma Receptor II A
Entrez Gene IDs
2212 (Human); 14130 (Mouse); 116591 (Rat); 102143568 (Cynomolgus Monkey)
Alternate Names
CD32 antigen; CD32a; CD32MGC23887; CDw32fc-gamma-RIIa; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32); Fc gamma RIIA; FCG2; Fc-gamma RII-a; Fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; IGFR2MGC30032; IgG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a

Citations for Recombinant Human Fc gamma RIIA/CD32a (R167) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus
    Authors: PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass
    J. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  2. Development and evaluation of antibody-capture immunoassays for detection of Lassa virus nucleoprotein-specific immunoglobulin M and G
    Authors: M Gabriel, DI Adomeh, J Ehimuan, J Oyakhilome, EO Omomoh, Y Ighodalo, T Olokor, K Bonney, M Pahlmann, P Emmerich, M Lelke, L Brunotte, S Ölschläger, C Thomé-Bold, B Becker-Zia, C Busch, I Odia, E Ogbaini-Em, PO Okokhere, SA Okogbenin, GO Akpede, H Schmitz, DA Asogun, S Günther
    PLoS Negl Trop Dis, 2018;12(3):e0006361.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  3. Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey
    Authors: T Oshima, H Miyashita, Y Ishimura, Y Ito, Y Tanaka, A Hori, T Kokubo, T Kurokawa
    PLoS ONE, 2018;13(5):e0196422.
    Applications: Bioassay
  4. Enzymatic inactivation of endogenous IgG by IdeS enhances�therapeutic antibody efficacy
    Authors: S Järnum, A Runström, R Bockermann, L Winstedt, M Crispin, C Kjellman
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Types: Antibody
    Applications: Bioassay
  5. Anti-CD20 Antibody with Multimerized Fc Domains: A Novel Strategy To Deplete B Cells and Augment Treatment of Autoimmune Disease.
    Authors: Zhang X, Olsen H, Chen S, So E, Zhou H, Burch E, Merigeon E, Block D, Strome S
    J Immunol, 2016;196(3):1165-76.
    Species: N/A
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  6. GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins.
    Authors: Meuris L, Santens F, Elson G, Festjens N, Boone M, Dos Santos A, Devos S, Rousseau F, Plets E, Houthuys E, Malinge P, Magistrelli G, Cons L, Chatel L, Devreese B, Callewaert N
    Nat Biotechnol, 2014;32(5):485-9.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  7. Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases.
    Authors: Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A
    J Immunol, 2014;192(9):4083-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. Osteoclast inhibitory peptide-1 binding to the Fc gammaRIIB inhibits osteoclast differentiation.
    Authors: Shanmugarajan S, Beeson CC, Reddy SV
    Endocrinology, 2010;151(9):4389-99.
    Species: Mouse
    Sample Types: Peptide
    Applications: Surface Plasmon Resonance
  9. Alpha-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8.
    Authors: Bergin DA, Reeves EP, Meleady P, Henry M, McElvaney OJ, Carroll TP, Condron C, Chotirmall SH, Clynes M, O'Neill SJ, McElvaney NG
    J. Clin. Invest., 2010;120(12):4236-50.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)

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