Recombinant Human FLAG-WWP1 Protein, CF

R&D Systems | Catalog # E3-232

R&D Systems
Discontinued Product
E3-232 has been discontinued. View all WWP1 products.

Key Product Details

Source

T. ni (baculovirus)

Accession Number

Q9H0M0

Applications

Bioactivity
View all WWP1 Proteins and Enzymes »

Product Specifications

Source

Trichoplusia ni, T. ni (baculovirus)-derived human WWP1 protein
2X FLAG ™ tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

107 kDa

Activity

Recombinant Human WWP1 is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.

Formulation, Preparation, and Storage

E3-232
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 48 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: WWP1

WWP1 belongs to a family of NEDD4-like proteins, which are HECT-class E3 ubiquitin ligase enzymes.  Human family members include NEDD4, NEDD4-2, ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDDL1 and NEDDL2.  With a predicted molecular weight of 105 kDa, WWP1 is 922 amino acid residues long and is 90% identical to the murine ortholog.  WWP1 reportedly ubiquitinates and promotes proteasome-dependent degradation of two c-ErbB-4 isoforms, the transcription factors KLF2 and KLF5, TP63, and a variety of other proteins.  WWP1 adopts an autoinhibited structure in which its multiple WW domains sequester the HECT domain.  Importantly, mutations in the WW domains of this ligase have been found in certain human cancers and have been shown to derepress WWP1 ligase activity in cell-based and in vitro assays.  This recombinant protein contains and N-terminal 2X FLAG tag and amino acids 2-922 (UniProt Q9H0M0)

References

  1. Komuro A. et al. (2004) Oncogene  23: 6914
  2. Seo S.R. et al. (2004) EMBO J.  23: 3780
  3. Verdecia M.A. et al. (2003) Mol. Cell  11: 249
  4. Wang Z et al. (2019) Nat. Comm.  10: 3162

Long Name

WW Domain Containing E3 Ubiquitin Protein Ligase 1

Alternate Names

AIP5, HSDRP1, Tiul11

Entrez Gene IDs

11059 (Human); 107568 (Mouse); 297930 (Rat)

Gene Symbol

WWP1

UniProt

Q9H0M0

Additional WWP1 Products

Product Documents for Recombinant Human FLAG-WWP1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human FLAG-WWP1 Protein, CF

For research use only

Related Research Areas

E3 Ubiquitin Ligases
Notch Pathway

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