Recombinant Human His10-USP47 Protein, CF

R&D Systems | Catalog # E-626

R&D Systems
Discontinued Product
E-626 has been discontinued. View all USP47 products.

Key Product Details

Source

Sf 21 (baculovirus)

Applications

Bioactivity
View all USP47 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP47 protein
Contains an N-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

160 kDa

Activity

Recombinant Human USP47 is a Ubiquitin deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial concentration of 10-100 nM USP47 when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine (U-555) as a substrate.

Reviewed Applications

Read 1 review rated 5 using E-626 in the following applications:

Formulation, Preparation, and Storage

E-626
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP47

Ubiquitin carboxyl-terminal hydrolase 47 (USP47) is a C19 type peptidase with a predicted molecular weight of 157 kDa.  The human protein shares 95% and 96% amino acid sequence identity with its mouse and rat orthologs, respectively.  USP47 has been reported to play roles in inflammasome activation and colon cancer progression.  This recombinant protein contains an n-terminal 10-His tag.

References

  1. Palazon-Riquelme, P. et al.  (2018) EMBO Rep.  doi: 10.15252/embr.201744766
  2. Yu, L. et al.  (2019) Cancer Letters  doi: 10.1016/j.canlet.2019.01.039

Long Name

Ubiquitin Specific Protease 47

Alternate Names

TRFP

Entrez Gene IDs

55031 (Human); 74996 (Mouse)

Gene Symbol

USP47

Additional USP47 Products

Product Documents for Recombinant Human His10-USP47 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His10-USP47 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Customer Reviews for Recombinant Human His10-USP47 Protein, CF (1)

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  • Recombinant Human His10-USP47 Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/21/2021

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