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Recombinant Human His10-USP47 Protein, CF

Catalog #: E-626 1 Review Datasheet / COA / SDS

Discontinued Product

E-626 has been discontinued.
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Recombinant Human His10-USP47 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human USP47 is a Ubiquitin deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial concentration of 10-100 nM USP47 when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine (U-555) as a substrate.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP47 protein
Contains an N-terminal 10-His tag
Predicted Molecular Mass
160 kDa

Product Datasheets

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E-626

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-626

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP47

Ubiquitin carboxyl-terminal hydrolase 47 (USP47) is a C19 type peptidase with a predicted molecular weight of 157 kDa.  The human protein shares 95% and 96% amino acid sequence identity with its mouse and rat orthologs, respectively.  USP47 has been reported to play roles in inflammasome activation and colon cancer progression.  This recombinant protein contains an n-terminal 10-His tag.

References
  1. Palazon-Riquelme, P. et al.  (2018) EMBO Rep.  doi: 10.15252/embr.201744766
  2. Yu, L. et al.  (2019) Cancer Letters  doi: 10.1016/j.canlet.2019.01.039
Long Name
Ubiquitin Specific Protease 47
Entrez Gene IDs
55031 (Human); 74996 (Mouse)
Alternate Names
Deubiquitinating enzyme 47; DKFZp686C13257; EC 3.4.19.12; FLJ20727; Trf (TATA binding protein-related factor)-proximal homolog (Drosophila); Trf (TATA binding protein-related factor)-proximal homolog; TRFP; ubiquitin carboxyl-terminal hydrolase 47; ubiquitin specific peptidase 47; ubiquitin specific protease 47; ubiquitin thioesterase 47; Ubiquitin thiolesterase 47; Ubiquitin-specific-processing protease 47; USP47

FAQs

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Reviews for Recombinant Human His10-USP47 Protein, CF

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Recombinant Human His10-USP47 Protein, CF
By Anonymous on 01/21/2021
Application: Enzymatic activity in vitro